1kut
From Proteopedia
(New page: 200px<br /><applet load="1kut" size="450" color="white" frame="true" align="right" spinBox="true" caption="1kut, resolution 2.20Å" /> '''Structural Genomics,...) |
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| - | [[Image:1kut.gif|left|200px]]<br /><applet load="1kut" size=" | + | [[Image:1kut.gif|left|200px]]<br /><applet load="1kut" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1kut, resolution 2.20Å" /> | caption="1kut, resolution 2.20Å" /> | ||
'''Structural Genomics, Protein TM1243, (SAICAR synthetase)'''<br /> | '''Structural Genomics, Protein TM1243, (SAICAR synthetase)'''<br /> | ||
==Overview== | ==Overview== | ||
| - | As a part of a structural genomics program, the 2.2 angstroms resolution | + | As a part of a structural genomics program, the 2.2 angstroms resolution crystal structure of the PurC gene product from Thermotoga maritima has been solved. This 26.2 kDa protein belongs to the phophoribosylaminoimidazole-succinocarboxamide or SAICAR synthase family of enzymes, the members of which are involved in de novo purine biosynthesis. SAICAR synthase can be divided into three subdomains: two alpha+beta regions exhibiting structural homology with ATP-binding proteins and a carboxy-terminal subdomain of two alpha-helices. The asymmetric unit contains two copies of the protein which are covalently linked by a disulfide bond between Cys126(A) and Cys126(B). This 230-amino-acid protein exhibits high structural homology with SAICAR synthase from baker's yeast. The protein structure is described and compared with that of the ATP-SAICAR synthase complex from yeast. |
==About this Structure== | ==About this Structure== | ||
| - | 1KUT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Active as [http://en.wikipedia.org/wiki/Phosphoribosylaminoimidazolesuccinocarboxamide_synthase Phosphoribosylaminoimidazolesuccinocarboxamide synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.6 6.3.2.6] Full crystallographic information is available from [http:// | + | 1KUT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Active as [http://en.wikipedia.org/wiki/Phosphoribosylaminoimidazolesuccinocarboxamide_synthase Phosphoribosylaminoimidazolesuccinocarboxamide synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.6 6.3.2.6] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KUT OCA]. |
==Reference== | ==Reference== | ||
| - | Structure of SAICAR synthase from Thermotoga maritima at 2.2 angstroms reveals an unusual covalent dimer., Zhang R, Skarina T, Evdokimova E, Edwards A, Savchenko A, Laskowski R, Cuff ME, Joachimiak A, Acta | + | Structure of SAICAR synthase from Thermotoga maritima at 2.2 angstroms reveals an unusual covalent dimer., Zhang R, Skarina T, Evdokimova E, Edwards A, Savchenko A, Laskowski R, Cuff ME, Joachimiak A, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Apr 1;62(Pt, 4):335-9. Epub 2006 Mar 25. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16582479 16582479] |
[[Category: Phosphoribosylaminoimidazolesuccinocarboxamide synthase]] | [[Category: Phosphoribosylaminoimidazolesuccinocarboxamide synthase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: Edwards, A.]] | [[Category: Edwards, A.]] | ||
[[Category: Joachimiak, A.]] | [[Category: Joachimiak, A.]] | ||
| - | [[Category: MCSG, Midwest | + | [[Category: MCSG, Midwest Center for Structural Genomics.]] |
[[Category: Savchenko, A.]] | [[Category: Savchenko, A.]] | ||
[[Category: Skarina, T.]] | [[Category: Skarina, T.]] | ||
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[[Category: structural genomics]] | [[Category: structural genomics]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:38:06 2008'' |
Revision as of 11:38, 21 February 2008
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Structural Genomics, Protein TM1243, (SAICAR synthetase)
Overview
As a part of a structural genomics program, the 2.2 angstroms resolution crystal structure of the PurC gene product from Thermotoga maritima has been solved. This 26.2 kDa protein belongs to the phophoribosylaminoimidazole-succinocarboxamide or SAICAR synthase family of enzymes, the members of which are involved in de novo purine biosynthesis. SAICAR synthase can be divided into three subdomains: two alpha+beta regions exhibiting structural homology with ATP-binding proteins and a carboxy-terminal subdomain of two alpha-helices. The asymmetric unit contains two copies of the protein which are covalently linked by a disulfide bond between Cys126(A) and Cys126(B). This 230-amino-acid protein exhibits high structural homology with SAICAR synthase from baker's yeast. The protein structure is described and compared with that of the ATP-SAICAR synthase complex from yeast.
About this Structure
1KUT is a Single protein structure of sequence from Thermotoga maritima. Active as Phosphoribosylaminoimidazolesuccinocarboxamide synthase, with EC number 6.3.2.6 Full crystallographic information is available from OCA.
Reference
Structure of SAICAR synthase from Thermotoga maritima at 2.2 angstroms reveals an unusual covalent dimer., Zhang R, Skarina T, Evdokimova E, Edwards A, Savchenko A, Laskowski R, Cuff ME, Joachimiak A, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Apr 1;62(Pt, 4):335-9. Epub 2006 Mar 25. PMID:16582479
Page seeded by OCA on Thu Feb 21 13:38:06 2008
Categories: Phosphoribosylaminoimidazolesuccinocarboxamide synthase | Single protein | Thermotoga maritima | Beasley, S. | Edwards, A. | Joachimiak, A. | MCSG, Midwest Center for Structural Genomics. | Savchenko, A. | Skarina, T. | Zhang, R. | Mcsg | Midwest center for structural genomics | Protein structure initiative | Psi | Saicar synthetase | Structural genomics
