3ran
From Proteopedia
(New page: 200px<br /><applet load="3ran" size="450" color="white" frame="true" align="right" spinBox="true" caption="3ran, resolution 2.15Å" /> '''CANINE GDP-RAN Q69L ...) |
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| - | [[Image:3ran.jpg|left|200px]]<br /><applet load="3ran" size=" | + | [[Image:3ran.jpg|left|200px]]<br /><applet load="3ran" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="3ran, resolution 2.15Å" /> | caption="3ran, resolution 2.15Å" /> | ||
'''CANINE GDP-RAN Q69L MUTANT'''<br /> | '''CANINE GDP-RAN Q69L MUTANT'''<br /> | ||
==Overview== | ==Overview== | ||
| - | We report the 2.3 A resolution X-ray crystal structure of the GDP-bound | + | We report the 2.3 A resolution X-ray crystal structure of the GDP-bound form of the RanQ69L mutant that is used extensively in studies of nucleocytoplasmic transport and cell-cycle progression. When the structure of GDP-RanQ69L from monoclinic crystals with P21 symmetry was compared with the structure of wild-type Ran obtained from monoclinic crystals, the Q69L mutant showed a large conformational change in residues 68-74, which are in the switch II region of the molecule which changes conformation in response to nucleotide state and which forms the major interaction interface with nuclear transport factor 2 (NTF2, sometimes called p10). This conformational change alters the positions of key residues such as Lys71, Phe72 and Arg76 that are crucial for the interaction of GDP-Ran with NTF2 and indeed, solution binding studies were unable to detect any interaction between NTF2 and GDP-RanQ69L under conditions where GDP-Ran bound effectively. This interaction between NTF2 and GDP-Ran is required for efficient nuclear protein import and may function between the docking and translocation steps of the pathway. |
==About this Structure== | ==About this Structure== | ||
| - | 3RAN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Canis_lupus_familiaris Canis lupus familiaris] with MG and GDP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 3RAN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Canis_lupus_familiaris Canis lupus familiaris] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=GDP:'>GDP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RAN OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Canis lupus familiaris]] | [[Category: Canis lupus familiaris]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Kent, H | + | [[Category: Kent, H M.]] |
| - | [[Category: Mccoy, A | + | [[Category: Mccoy, A J.]] |
[[Category: Stewart, M.]] | [[Category: Stewart, M.]] | ||
[[Category: GDP]] | [[Category: GDP]] | ||
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[[Category: nuclear transport]] | [[Category: nuclear transport]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:11:07 2008'' |
Revision as of 17:11, 21 February 2008
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CANINE GDP-RAN Q69L MUTANT
Overview
We report the 2.3 A resolution X-ray crystal structure of the GDP-bound form of the RanQ69L mutant that is used extensively in studies of nucleocytoplasmic transport and cell-cycle progression. When the structure of GDP-RanQ69L from monoclinic crystals with P21 symmetry was compared with the structure of wild-type Ran obtained from monoclinic crystals, the Q69L mutant showed a large conformational change in residues 68-74, which are in the switch II region of the molecule which changes conformation in response to nucleotide state and which forms the major interaction interface with nuclear transport factor 2 (NTF2, sometimes called p10). This conformational change alters the positions of key residues such as Lys71, Phe72 and Arg76 that are crucial for the interaction of GDP-Ran with NTF2 and indeed, solution binding studies were unable to detect any interaction between NTF2 and GDP-RanQ69L under conditions where GDP-Ran bound effectively. This interaction between NTF2 and GDP-Ran is required for efficient nuclear protein import and may function between the docking and translocation steps of the pathway.
About this Structure
3RAN is a Single protein structure of sequence from Canis lupus familiaris with and as ligands. Full crystallographic information is available from OCA.
Reference
The structure of the Q69L mutant of GDP-Ran shows a major conformational change in the switch II loop that accounts for its failure to bind nuclear transport factor 2 (NTF2)., Stewart M, Kent HM, McCoy AJ, J Mol Biol. 1998 Dec 18;284(5):1517-27. PMID:9878368
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