3std
From Proteopedia
(New page: 200px<br /><applet load="3std" size="450" color="white" frame="true" align="right" spinBox="true" caption="3std, resolution 1.65Å" /> '''SCYTALONE DEHYDRATAS...) |
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| - | [[Image:3std.gif|left|200px]]<br /><applet load="3std" size=" | + | [[Image:3std.gif|left|200px]]<br /><applet load="3std" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="3std, resolution 1.65Å" /> | caption="3std, resolution 1.65Å" /> | ||
'''SCYTALONE DEHYDRATASE AND CYANOCINNOLINE INHIBITOR'''<br /> | '''SCYTALONE DEHYDRATASE AND CYANOCINNOLINE INHIBITOR'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Scytalone dehydratase (SD) is a molecular target of inhibitor design | + | Scytalone dehydratase (SD) is a molecular target of inhibitor design efforts aimed at protecting rice plants from the fungal disease caused by Magnaporthe grisea. As determined from X-ray diffraction data of an SD-inhibitor complex [Lundqvist et al. (1994) Structure (London) 2, 937-944], there is an extended hydrogen-bonding network between protein side chains, the inhibitor, and two bound water molecules. From models of SD complexed to quinazoline and benztriazine inhibitors, a new class of potent SD inhibitors involving the displacement of an active-site water molecule were designed. We were able to increase inhibitory potency by synthesizing compounds with a nitrile functionality displayed into the space occupied by one of the crystallographic water molecules. Sixteen inhibitors are compared. The net conversion of potent quinazoline and benztriazine inhibitors to cyanoquinolines and cyanocinnolines increased binding potency 2-20-fold. Replacement of the nitrile with a hydrogen atom lowered binding affinity 100-30,000-fold. X-ray crystallographic data at 1.65 A resolution on a SD-inhibitor complex confirmed that the nitrile functionality displaced the water molecule as intended and that a favorable orientation was created with tyrosines 30 and 50 which had been part of the hydrogen-bonding network with the water molecule. Additional data on inhibitors presented herein reveals the importance of two hydrogen-bonding networks toward inhibitory potency: one between Asn131 and an appropriately positioned inhibitor heteroatom and one between a bound water molecule and a second inhibitor heteroatom. |
==About this Structure== | ==About this Structure== | ||
| - | 3STD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Magnaporthe_grisea Magnaporthe grisea] with CA and MQ0 as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Scytalone_dehydratase Scytalone dehydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.94 4.2.1.94] Full crystallographic information is available from [http:// | + | 3STD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Magnaporthe_grisea Magnaporthe grisea] with <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=MQ0:'>MQ0</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Scytalone_dehydratase Scytalone dehydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.94 4.2.1.94] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3STD OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Scytalone dehydratase]] | [[Category: Scytalone dehydratase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Basarab, G | + | [[Category: Basarab, G S.]] |
| - | [[Category: Chen, J | + | [[Category: Chen, J M.]] |
| - | [[Category: Jordan, D | + | [[Category: Jordan, D B.]] |
[[Category: Wawrzak, Z.]] | [[Category: Wawrzak, Z.]] | ||
| - | [[Category: Xu, S | + | [[Category: Xu, S L.]] |
[[Category: CA]] | [[Category: CA]] | ||
[[Category: MQ0]] | [[Category: MQ0]] | ||
[[Category: dehydratase]] | [[Category: dehydratase]] | ||
| - | [[Category: ec 4 | + | [[Category: ec 4 2.1 94]] |
[[Category: fungal melanin]] | [[Category: fungal melanin]] | ||
[[Category: lyase]] | [[Category: lyase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:11:24 2008'' |
Revision as of 17:11, 21 February 2008
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SCYTALONE DEHYDRATASE AND CYANOCINNOLINE INHIBITOR
Overview
Scytalone dehydratase (SD) is a molecular target of inhibitor design efforts aimed at protecting rice plants from the fungal disease caused by Magnaporthe grisea. As determined from X-ray diffraction data of an SD-inhibitor complex [Lundqvist et al. (1994) Structure (London) 2, 937-944], there is an extended hydrogen-bonding network between protein side chains, the inhibitor, and two bound water molecules. From models of SD complexed to quinazoline and benztriazine inhibitors, a new class of potent SD inhibitors involving the displacement of an active-site water molecule were designed. We were able to increase inhibitory potency by synthesizing compounds with a nitrile functionality displayed into the space occupied by one of the crystallographic water molecules. Sixteen inhibitors are compared. The net conversion of potent quinazoline and benztriazine inhibitors to cyanoquinolines and cyanocinnolines increased binding potency 2-20-fold. Replacement of the nitrile with a hydrogen atom lowered binding affinity 100-30,000-fold. X-ray crystallographic data at 1.65 A resolution on a SD-inhibitor complex confirmed that the nitrile functionality displaced the water molecule as intended and that a favorable orientation was created with tyrosines 30 and 50 which had been part of the hydrogen-bonding network with the water molecule. Additional data on inhibitors presented herein reveals the importance of two hydrogen-bonding networks toward inhibitory potency: one between Asn131 and an appropriately positioned inhibitor heteroatom and one between a bound water molecule and a second inhibitor heteroatom.
About this Structure
3STD is a Single protein structure of sequence from Magnaporthe grisea with and as ligands. Active as Scytalone dehydratase, with EC number 4.2.1.94 Full crystallographic information is available from OCA.
Reference
Structure-based design of potent inhibitors of scytalone dehydratase: displacement of a water molecule from the active site., Chen JM, Xu SL, Wawrzak Z, Basarab GS, Jordan DB, Biochemistry. 1998 Dec 22;37(51):17735-44. PMID:9922139
Page seeded by OCA on Thu Feb 21 19:11:24 2008
Categories: Magnaporthe grisea | Scytalone dehydratase | Single protein | Basarab, G S. | Chen, J M. | Jordan, D B. | Wawrzak, Z. | Xu, S L. | CA | MQ0 | Dehydratase | Ec 4 2.1 94 | Fungal melanin | Lyase
