3sxl
From Proteopedia
(New page: 200px<br /><applet load="3sxl" size="450" color="white" frame="true" align="right" spinBox="true" caption="3sxl, resolution 2.700Å" /> '''SEX-LETHAL RNA RECO...) |
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| - | [[Image:3sxl.gif|left|200px]]<br /><applet load="3sxl" size=" | + | [[Image:3sxl.gif|left|200px]]<br /><applet load="3sxl" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="3sxl, resolution 2.700Å" /> | caption="3sxl, resolution 2.700Å" /> | ||
'''SEX-LETHAL RNA RECOGNITION DOMAINS 1 AND 2 FROM DROSOPHILA MELANOGASTER'''<br /> | '''SEX-LETHAL RNA RECOGNITION DOMAINS 1 AND 2 FROM DROSOPHILA MELANOGASTER'''<br /> | ||
==Overview== | ==Overview== | ||
| - | By binding specific RNA transcripts, the Sex-lethal protein (SXL) governs | + | By binding specific RNA transcripts, the Sex-lethal protein (SXL) governs sexual differentiation and dosage compensation in Drosophila melanogaster. To investigate the basis for RNA binding specificity, we determined the crystal structure of the tandem RNA recognition motifs (RRMs) of SXL. Both RRMs adopt the canonical RRM fold, and the 10-residue, interdomain linker shows significant disorder. In contrast to the previously determined structure of the two-RRM fragment of heterogeneous nuclear ribonucleoprotein Al, SXL displays no interdomain contacts between RRMs. These results suggest that the SXL RRMs are flexibly tethered in solution, and RNA binding restricts the orientation of RRMs. Therefore, the observed specificity for single-stranded, U-rich sequences does not arise from a predefined, rigid architecture of the isolated SXL RRMs. |
==About this Structure== | ==About this Structure== | ||
| - | 3SXL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http:// | + | 3SXL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SXL OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Drosophila melanogaster]] | [[Category: Drosophila melanogaster]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Alber, T | + | [[Category: Alber, T C.]] |
| - | [[Category: Crowder, S | + | [[Category: Crowder, S M.]] |
[[Category: Kanaar, R.]] | [[Category: Kanaar, R.]] | ||
| - | [[Category: Rio, D | + | [[Category: Rio, D C.]] |
[[Category: rbd]] | [[Category: rbd]] | ||
[[Category: rna binding domain]] | [[Category: rna binding domain]] | ||
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[[Category: x chromosome dosage compensation]] | [[Category: x chromosome dosage compensation]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:11:25 2008'' |
Revision as of 17:11, 21 February 2008
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SEX-LETHAL RNA RECOGNITION DOMAINS 1 AND 2 FROM DROSOPHILA MELANOGASTER
Overview
By binding specific RNA transcripts, the Sex-lethal protein (SXL) governs sexual differentiation and dosage compensation in Drosophila melanogaster. To investigate the basis for RNA binding specificity, we determined the crystal structure of the tandem RNA recognition motifs (RRMs) of SXL. Both RRMs adopt the canonical RRM fold, and the 10-residue, interdomain linker shows significant disorder. In contrast to the previously determined structure of the two-RRM fragment of heterogeneous nuclear ribonucleoprotein Al, SXL displays no interdomain contacts between RRMs. These results suggest that the SXL RRMs are flexibly tethered in solution, and RNA binding restricts the orientation of RRMs. Therefore, the observed specificity for single-stranded, U-rich sequences does not arise from a predefined, rigid architecture of the isolated SXL RRMs.
About this Structure
3SXL is a Single protein structure of sequence from Drosophila melanogaster. Full crystallographic information is available from OCA.
Reference
Absence of interdomain contacts in the crystal structure of the RNA recognition motifs of Sex-lethal., Crowder SM, Kanaar R, Rio DC, Alber T, Proc Natl Acad Sci U S A. 1999 Apr 27;96(9):4892-7. PMID:10220389
Page seeded by OCA on Thu Feb 21 19:11:25 2008
