1kzl
From Proteopedia
(New page: 200px<br /><applet load="1kzl" size="450" color="white" frame="true" align="right" spinBox="true" caption="1kzl, resolution 2.1Å" /> '''Riboflavin Synthase f...) |
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| - | [[Image:1kzl.jpg|left|200px]]<br /><applet load="1kzl" size=" | + | [[Image:1kzl.jpg|left|200px]]<br /><applet load="1kzl" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1kzl, resolution 2.1Å" /> | caption="1kzl, resolution 2.1Å" /> | ||
'''Riboflavin Synthase from S.pombe bound to Carboxyethyllumazine'''<br /> | '''Riboflavin Synthase from S.pombe bound to Carboxyethyllumazine'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Riboflavin synthase catalyzes the disproportionation of | + | Riboflavin synthase catalyzes the disproportionation of 6,7-dimethyl-8-ribityllumazine affording riboflavin and 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione. We have determined the structure of riboflavin synthase from Schizosaccharomyces pombe in complex with the substrate analog, 6-carboxyethyl-7-oxo-8-ribityllumazine at 2.1 A resolution. In contrast to the homotrimeric solution state of native riboflavin synthase, we found the enzyme to be monomeric in the crystal structure. Structural comparison of the riboflavin synthases of S. pombe and Escherichia coli suggests oligomer contact sites and delineates the catalytic site for dimerization of the substrate and subsequent fragmentation of the pentacyclic intermediate. The pentacyclic substrate dimer was modeled into the proposed active site, and its stereochemical features were determined. The model suggests that the substrate molecule at the C-terminal domain donates a four-carbon unit to the substrate molecule bound at the N-terminal domain of an adjacent subunit in the oligomer. |
==About this Structure== | ==About this Structure== | ||
| - | 1KZL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe] with HG and CRM as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Riboflavin_synthase Riboflavin synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.9 2.5.1.9] Full crystallographic information is available from [http:// | + | 1KZL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe] with <scene name='pdbligand=HG:'>HG</scene> and <scene name='pdbligand=CRM:'>CRM</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Riboflavin_synthase Riboflavin synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.9 2.5.1.9] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KZL OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Illarionov, B.]] | [[Category: Illarionov, B.]] | ||
[[Category: Kairies, N.]] | [[Category: Kairies, N.]] | ||
| - | [[Category: Schott, A | + | [[Category: Schott, A K.]] |
[[Category: Steinbacher, S.]] | [[Category: Steinbacher, S.]] | ||
[[Category: CRM]] | [[Category: CRM]] | ||
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[[Category: x-ray structure]] | [[Category: x-ray structure]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:39:40 2008'' |
Revision as of 11:39, 21 February 2008
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Riboflavin Synthase from S.pombe bound to Carboxyethyllumazine
Overview
Riboflavin synthase catalyzes the disproportionation of 6,7-dimethyl-8-ribityllumazine affording riboflavin and 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione. We have determined the structure of riboflavin synthase from Schizosaccharomyces pombe in complex with the substrate analog, 6-carboxyethyl-7-oxo-8-ribityllumazine at 2.1 A resolution. In contrast to the homotrimeric solution state of native riboflavin synthase, we found the enzyme to be monomeric in the crystal structure. Structural comparison of the riboflavin synthases of S. pombe and Escherichia coli suggests oligomer contact sites and delineates the catalytic site for dimerization of the substrate and subsequent fragmentation of the pentacyclic intermediate. The pentacyclic substrate dimer was modeled into the proposed active site, and its stereochemical features were determined. The model suggests that the substrate molecule at the C-terminal domain donates a four-carbon unit to the substrate molecule bound at the N-terminal domain of an adjacent subunit in the oligomer.
About this Structure
1KZL is a Single protein structure of sequence from Schizosaccharomyces pombe with and as ligands. Active as Riboflavin synthase, with EC number 2.5.1.9 Full crystallographic information is available from OCA.
Reference
Studies on the reaction mechanism of riboflavin synthase: X-ray crystal structure of a complex with 6-carboxyethyl-7-oxo-8-ribityllumazine., Gerhardt S, Schott AK, Kairies N, Cushman M, Illarionov B, Eisenreich W, Bacher A, Huber R, Steinbacher S, Fischer M, Structure. 2002 Oct;10(10):1371-81. PMID:12377123
Page seeded by OCA on Thu Feb 21 13:39:40 2008
Categories: Riboflavin synthase | Schizosaccharomyces pombe | Single protein | Bacher, A. | Cushman, M. | Eisenreich, W. | Fischer, M. | Gerhardt, S. | Huber, R. | Illarionov, B. | Kairies, N. | Schott, A K. | Steinbacher, S. | CRM | HG | Biosynthesis of riboflavin | Ligand binding | X-ray structure
