1l2m
From Proteopedia
(New page: 200px<br /><applet load="1l2m" size="450" color="white" frame="true" align="right" spinBox="true" caption="1l2m" /> '''Minimized Average Structure of the N-termina...) |
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'''Minimized Average Structure of the N-terminal, DNA-binding domain of the replication initiation protein from a geminivirus (Tomato yellow leaf curl virus-Sardinia)'''<br /> | '''Minimized Average Structure of the N-terminal, DNA-binding domain of the replication initiation protein from a geminivirus (Tomato yellow leaf curl virus-Sardinia)'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Rolling circle replication is a mechanism for copying single-stranded | + | Rolling circle replication is a mechanism for copying single-stranded genomes by means of double-stranded intermediates. A multifunctional replication initiator protein (Rep) is indispensable for the precise initiation and termination of this process. Despite the ubiquitous presence and fundamental importance of rolling circle replication elements, structural information on their respective replication initiators is still missing. Here we present the solution NMR structure of the catalytic domain of Rep, the initiator protein of tomato yellow leaf curl virus. It is composed of a central five-stranded anti-parallel beta-sheet, flanked by a small two-stranded beta-sheet, a beta-hairpin and two alpha-helices. Surprisingly, the structure reveals that the catalytic Rep domain is related to a large group of proteins that bind RNA or DNA. Identification of Rep as resembling the family of ribonucleoprotein/RNA-recognition motif fold proteins establishes a structure-based evolutionary link between RNA binding proteins, splicing factors, and replication initiators of prokaryotic and eukaryotic single-stranded DNA elements and mammalian DNA tumor viruses. |
==About this Structure== | ==About this Structure== | ||
| - | 1L2M is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Tomato_yellow_leaf_curl_sardinia_virus Tomato yellow leaf curl sardinia virus]. Full crystallographic information is available from [http:// | + | 1L2M is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Tomato_yellow_leaf_curl_sardinia_virus Tomato yellow leaf curl sardinia virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L2M OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Campos-Olivas, R.]] | [[Category: Campos-Olivas, R.]] | ||
[[Category: Clerot, D.]] | [[Category: Clerot, D.]] | ||
| - | [[Category: Gronenborn, A | + | [[Category: Gronenborn, A M.]] |
[[Category: Gronenborn, B.]] | [[Category: Gronenborn, B.]] | ||
| - | [[Category: Louis, J | + | [[Category: Louis, J M.]] |
[[Category: a+b fold]] | [[Category: a+b fold]] | ||
[[Category: rbd-like fold]] | [[Category: rbd-like fold]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:40:40 2008'' |
Revision as of 11:40, 21 February 2008
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Minimized Average Structure of the N-terminal, DNA-binding domain of the replication initiation protein from a geminivirus (Tomato yellow leaf curl virus-Sardinia)
Overview
Rolling circle replication is a mechanism for copying single-stranded genomes by means of double-stranded intermediates. A multifunctional replication initiator protein (Rep) is indispensable for the precise initiation and termination of this process. Despite the ubiquitous presence and fundamental importance of rolling circle replication elements, structural information on their respective replication initiators is still missing. Here we present the solution NMR structure of the catalytic domain of Rep, the initiator protein of tomato yellow leaf curl virus. It is composed of a central five-stranded anti-parallel beta-sheet, flanked by a small two-stranded beta-sheet, a beta-hairpin and two alpha-helices. Surprisingly, the structure reveals that the catalytic Rep domain is related to a large group of proteins that bind RNA or DNA. Identification of Rep as resembling the family of ribonucleoprotein/RNA-recognition motif fold proteins establishes a structure-based evolutionary link between RNA binding proteins, splicing factors, and replication initiators of prokaryotic and eukaryotic single-stranded DNA elements and mammalian DNA tumor viruses.
About this Structure
1L2M is a Single protein structure of sequence from Tomato yellow leaf curl sardinia virus. Full crystallographic information is available from OCA.
Reference
The structure of a replication initiator unites diverse aspects of nucleic acid metabolism., Campos-Olivas R, Louis JM, Clerot D, Gronenborn B, Gronenborn AM, Proc Natl Acad Sci U S A. 2002 Aug 6;99(16):10310-5. Epub 2002 Jul 18. PMID:12130667
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