1l5y
From Proteopedia
(New page: 200px<br /><applet load="1l5y" size="450" color="white" frame="true" align="right" spinBox="true" caption="1l5y, resolution 2.10Å" /> '''CRYSTAL STRUCTURE OF...) |
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| - | [[Image:1l5y.jpg|left|200px]]<br /><applet load="1l5y" size=" | + | [[Image:1l5y.jpg|left|200px]]<br /><applet load="1l5y" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1l5y, resolution 2.10Å" /> | caption="1l5y, resolution 2.10Å" /> | ||
'''CRYSTAL STRUCTURE OF MG2+ / BEF3-BOUND RECEIVER DOMAIN OF SINORHIZOBIUM MELILOTI DCTD'''<br /> | '''CRYSTAL STRUCTURE OF MG2+ / BEF3-BOUND RECEIVER DOMAIN OF SINORHIZOBIUM MELILOTI DCTD'''<br /> | ||
==Overview== | ==Overview== | ||
| - | A Crystallogral structure is described for the Mg2+-BeF3--bound receiver | + | A Crystallogral structure is described for the Mg2+-BeF3--bound receiver domain of Sinorhizobium meliloti DctD bearing amino acid substitution E121K. Differences between the apo- and ligand-bound active sites are similar to those reported for other receiver domains. However, the off and on states of the DctD receiver domain are characterized by dramatically different dimeric structures, which supports the following hypothesis of signal transduction. In the off state, the receiver domain and coiled-coil linker form a dimer that inhibits oligomerization of the AAA+ ATPase domain. In this conformation, the receiver domain cannot be phosphorylated or bind Mg2+ and BeF3-. Instead, these modifications stabilize an alternative dimeric conformation that repositions the subunits by approximately 20 A, thus replacing the a4-b5-a5 interface with an a4-b5 interface. Reoriented receiver domains permit the ATPase domain to oligomerize and stimulate open complex formation by the s54 form of RNA polymerase. NtrC, which shares 38% sequence identity with DctD, works differently. Its activated receiver domain must facilitate oligomerization of its ATPase domain. Significant differences exist in the signaling surfaces of the DctD and NtrC receiver domains that may help explain how triggering the common two-component switch can variously regulate assembly of a AAA+ ATPase domain. |
==About this Structure== | ==About this Structure== | ||
| - | 1L5Y is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sinorhizobium_meliloti Sinorhizobium meliloti] with MG, SO4, BEF, BF2, BF4 and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1L5Y is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sinorhizobium_meliloti Sinorhizobium meliloti] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=BEF:'>BEF</scene>, <scene name='pdbligand=BF2:'>BF2</scene>, <scene name='pdbligand=BF4:'>BF4</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L5Y OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Sinorhizobium meliloti]] | [[Category: Sinorhizobium meliloti]] | ||
| - | [[Category: Jones, A | + | [[Category: Jones, A D.]] |
[[Category: Meyer, M.]] | [[Category: Meyer, M.]] | ||
| - | [[Category: Nixon, B | + | [[Category: Nixon, B T.]] |
[[Category: Park, S.]] | [[Category: Park, S.]] | ||
| - | [[Category: Yennawar, H | + | [[Category: Yennawar, H P.]] |
| - | [[Category: Yennawar, N | + | [[Category: Yennawar, N H.]] |
[[Category: BEF]] | [[Category: BEF]] | ||
[[Category: BF2]] | [[Category: BF2]] | ||
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[[Category: beryllofluoride bound two component receiver domain]] | [[Category: beryllofluoride bound two component receiver domain]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:41:42 2008'' |
Revision as of 11:41, 21 February 2008
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CRYSTAL STRUCTURE OF MG2+ / BEF3-BOUND RECEIVER DOMAIN OF SINORHIZOBIUM MELILOTI DCTD
Overview
A Crystallogral structure is described for the Mg2+-BeF3--bound receiver domain of Sinorhizobium meliloti DctD bearing amino acid substitution E121K. Differences between the apo- and ligand-bound active sites are similar to those reported for other receiver domains. However, the off and on states of the DctD receiver domain are characterized by dramatically different dimeric structures, which supports the following hypothesis of signal transduction. In the off state, the receiver domain and coiled-coil linker form a dimer that inhibits oligomerization of the AAA+ ATPase domain. In this conformation, the receiver domain cannot be phosphorylated or bind Mg2+ and BeF3-. Instead, these modifications stabilize an alternative dimeric conformation that repositions the subunits by approximately 20 A, thus replacing the a4-b5-a5 interface with an a4-b5 interface. Reoriented receiver domains permit the ATPase domain to oligomerize and stimulate open complex formation by the s54 form of RNA polymerase. NtrC, which shares 38% sequence identity with DctD, works differently. Its activated receiver domain must facilitate oligomerization of its ATPase domain. Significant differences exist in the signaling surfaces of the DctD and NtrC receiver domains that may help explain how triggering the common two-component switch can variously regulate assembly of a AAA+ ATPase domain.
About this Structure
1L5Y is a Single protein structure of sequence from Sinorhizobium meliloti with , , , , and as ligands. Full crystallographic information is available from OCA.
Reference
Two-component signaling in the AAA + ATPase DctD: binding Mg2+ and BeF3- selects between alternate dimeric states of the receiver domain., Park S, Meyer M, Jones AD, Yennawar HP, Yennawar NH, Nixon BT, FASEB J. 2002 Dec;16(14):1964-6. Epub 2002 Oct 4. PMID:12368235
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