1l6o
From Proteopedia
(New page: 200px<br /><applet load="1l6o" size="450" color="white" frame="true" align="right" spinBox="true" caption="1l6o, resolution 2.200Å" /> '''XENOPUS DISHEVELLED...) |
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| - | [[Image:1l6o.gif|left|200px]]<br /><applet load="1l6o" size=" | + | [[Image:1l6o.gif|left|200px]]<br /><applet load="1l6o" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1l6o, resolution 2.200Å" /> | caption="1l6o, resolution 2.200Å" /> | ||
'''XENOPUS DISHEVELLED PDZ DOMAIN'''<br /> | '''XENOPUS DISHEVELLED PDZ DOMAIN'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Dapper was isolated in a screen for proteins interacting with Dishevelled, a key factor in Wnt signaling. Dapper and Dishevelled colocalize | + | Dapper was isolated in a screen for proteins interacting with Dishevelled, a key factor in Wnt signaling. Dapper and Dishevelled colocalize intracellularly and form a complex with Axin, GSK-3, CKI, and beta-catenin. Overexpression of Dapper increases Axin and GSK-3 in this complex, resulting in decreased soluble beta-catenin and decreased activation of beta-catenin-responsive genes. Dapper also inhibits activation by Dishevelled of c-Jun N-terminal kinase (JNK), a component of beta-catenin-independent Frizzled signaling. Inhibition of Dapper activates both beta-catenin-responsive genes and an AP1-responsive promoter, demonstrating that Dapper is a general Dishevelled antagonist. Depletion of maternal Dapper RNA from Xenopus embryos results in loss of notochord and head structures, demonstrating that Dapper is required for normal vertebrate development. |
==About this Structure== | ==About this Structure== | ||
| - | 1L6O is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis]. Full crystallographic information is available from [http:// | + | 1L6O is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L6O OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Xenopus laevis]] | [[Category: Xenopus laevis]] | ||
| - | [[Category: Cheyette, B | + | [[Category: Cheyette, B N.R.]] |
[[Category: Earnest, T.]] | [[Category: Earnest, T.]] | ||
| - | [[Category: Fox, E | + | [[Category: Fox, E P.]] |
[[Category: Khlebtsova, N.]] | [[Category: Khlebtsova, N.]] | ||
| - | [[Category: Miller, J | + | [[Category: Miller, J R.]] |
| - | [[Category: Moon, R | + | [[Category: Moon, R T.]] |
| - | [[Category: Sheldahl, L | + | [[Category: Sheldahl, L C.]] |
| - | [[Category: Takemaru, K | + | [[Category: Takemaru, K I.]] |
| - | [[Category: Waxman, J | + | [[Category: Waxman, J S.]] |
[[Category: dishevelled]] | [[Category: dishevelled]] | ||
[[Category: molecular recognition]] | [[Category: molecular recognition]] | ||
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[[Category: wnt pathway]] | [[Category: wnt pathway]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:41:55 2008'' |
Revision as of 11:41, 21 February 2008
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XENOPUS DISHEVELLED PDZ DOMAIN
Overview
Dapper was isolated in a screen for proteins interacting with Dishevelled, a key factor in Wnt signaling. Dapper and Dishevelled colocalize intracellularly and form a complex with Axin, GSK-3, CKI, and beta-catenin. Overexpression of Dapper increases Axin and GSK-3 in this complex, resulting in decreased soluble beta-catenin and decreased activation of beta-catenin-responsive genes. Dapper also inhibits activation by Dishevelled of c-Jun N-terminal kinase (JNK), a component of beta-catenin-independent Frizzled signaling. Inhibition of Dapper activates both beta-catenin-responsive genes and an AP1-responsive promoter, demonstrating that Dapper is a general Dishevelled antagonist. Depletion of maternal Dapper RNA from Xenopus embryos results in loss of notochord and head structures, demonstrating that Dapper is required for normal vertebrate development.
About this Structure
1L6O is a Protein complex structure of sequences from Xenopus laevis. Full crystallographic information is available from OCA.
Reference
Dapper, a Dishevelled-associated antagonist of beta-catenin and JNK signaling, is required for notochord formation., Cheyette BN, Waxman JS, Miller JR, Takemaru K, Sheldahl LC, Khlebtsova N, Fox EP, Earnest T, Moon RT, Dev Cell. 2002 Apr;2(4):449-61. PMID:11970895
Page seeded by OCA on Thu Feb 21 13:41:55 2008
