1l6r
From Proteopedia
(New page: 200px<br /><applet load="1l6r" size="450" color="white" frame="true" align="right" spinBox="true" caption="1l6r, resolution 1.4Å" /> '''Crystal Structure of ...) |
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| - | [[Image:1l6r.gif|left|200px]]<br /><applet load="1l6r" size=" | + | [[Image:1l6r.gif|left|200px]]<br /><applet load="1l6r" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1l6r, resolution 1.4Å" /> | caption="1l6r, resolution 1.4Å" /> | ||
'''Crystal Structure of Thermoplasma acidophilum 0175 (APC0014)'''<br /> | '''Crystal Structure of Thermoplasma acidophilum 0175 (APC0014)'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The protein TA0175 has a large number of sequence homologues, most of | + | The protein TA0175 has a large number of sequence homologues, most of which are annotated as unknown and a few as belonging to the haloacid dehalogenase superfamily, but has no known biological function. Using a combination of amino acid sequence analysis, three-dimensional crystal structure information, and kinetic analysis, we have characterized TA0175 as phosphoglycolate phosphatase from Thermoplasma acidophilum. The crystal structure of TA0175 revealed two distinct domains, a larger core domain and a smaller cap domain. The large domain is composed of a centrally located five-stranded parallel beta-sheet with strand order S10, S9, S8, S1, S2 and a small beta-hairpin, strands S3 and S4. This central sheet is flanked by a set of three alpha-helices on one side and two helices on the other. The smaller domain is composed of an open faced beta-sandwich represented by three antiparallel beta-strands, S5, S6, and S7, flanked by two oppositely oriented alpha-helices, H3 and H4. The topology of the large domain is conserved; however, structural variation is observed in the smaller domain among the different functional classes of the haloacid dehalogenase superfamily. Enzymatic assays on TA0175 revealed that this enzyme catalyzed the dephosphorylation of phosphoglycolate in vitro with similar kinetic properties seen for eukaryotic phosphoglycolate phosphatase. Activation by divalent cations, especially Mg2+, and competitive inhibition behavior with Cl- ions are similar between TA0175 and phosphoglycolate phosphatase. The experimental evidence presented for TA0175 is indicative of phosphoglycolate phosphatase. |
==About this Structure== | ==About this Structure== | ||
| - | 1L6R is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermoplasma_acidophilum Thermoplasma acidophilum] with CA and FMT as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1L6R is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermoplasma_acidophilum Thermoplasma acidophilum] with <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=FMT:'>FMT</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L6R OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Cheung, F.]] | [[Category: Cheung, F.]] | ||
[[Category: Christendat, D.]] | [[Category: Christendat, D.]] | ||
| - | [[Category: Edwards, A | + | [[Category: Edwards, A M.]] |
[[Category: Gu, J.]] | [[Category: Gu, J.]] | ||
[[Category: Joachimiak, A.]] | [[Category: Joachimiak, A.]] | ||
[[Category: Kim, Y.]] | [[Category: Kim, Y.]] | ||
| - | [[Category: MCSG, Midwest | + | [[Category: MCSG, Midwest Center for Structural Genomics.]] |
[[Category: Pennycooke, M.]] | [[Category: Pennycooke, M.]] | ||
[[Category: Xu, X.]] | [[Category: Xu, X.]] | ||
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[[Category: structural genomics]] | [[Category: structural genomics]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:41:55 2008'' |
Revision as of 11:41, 21 February 2008
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Crystal Structure of Thermoplasma acidophilum 0175 (APC0014)
Overview
The protein TA0175 has a large number of sequence homologues, most of which are annotated as unknown and a few as belonging to the haloacid dehalogenase superfamily, but has no known biological function. Using a combination of amino acid sequence analysis, three-dimensional crystal structure information, and kinetic analysis, we have characterized TA0175 as phosphoglycolate phosphatase from Thermoplasma acidophilum. The crystal structure of TA0175 revealed two distinct domains, a larger core domain and a smaller cap domain. The large domain is composed of a centrally located five-stranded parallel beta-sheet with strand order S10, S9, S8, S1, S2 and a small beta-hairpin, strands S3 and S4. This central sheet is flanked by a set of three alpha-helices on one side and two helices on the other. The smaller domain is composed of an open faced beta-sandwich represented by three antiparallel beta-strands, S5, S6, and S7, flanked by two oppositely oriented alpha-helices, H3 and H4. The topology of the large domain is conserved; however, structural variation is observed in the smaller domain among the different functional classes of the haloacid dehalogenase superfamily. Enzymatic assays on TA0175 revealed that this enzyme catalyzed the dephosphorylation of phosphoglycolate in vitro with similar kinetic properties seen for eukaryotic phosphoglycolate phosphatase. Activation by divalent cations, especially Mg2+, and competitive inhibition behavior with Cl- ions are similar between TA0175 and phosphoglycolate phosphatase. The experimental evidence presented for TA0175 is indicative of phosphoglycolate phosphatase.
About this Structure
1L6R is a Single protein structure of sequence from Thermoplasma acidophilum with and as ligands. Full crystallographic information is available from OCA.
Reference
Structure- and function-based characterization of a new phosphoglycolate phosphatase from Thermoplasma acidophilum., Kim Y, Yakunin AF, Kuznetsova E, Xu X, Pennycooke M, Gu J, Cheung F, Proudfoot M, Arrowsmith CH, Joachimiak A, Edwards AM, Christendat D, J Biol Chem. 2004 Jan 2;279(1):517-26. Epub 2003 Oct 10. PMID:14555659
Page seeded by OCA on Thu Feb 21 13:41:55 2008
Categories: Single protein | Thermoplasma acidophilum | Cheung, F. | Christendat, D. | Edwards, A M. | Gu, J. | Joachimiak, A. | Kim, Y. | MCSG, Midwest Center for Structural Genomics. | Pennycooke, M. | Xu, X. | CA | FMT | Hypothetical protein | Mcsg | Midwest center for structural genomics | Protein structure initiative | Psi | Putative hydrolase | Structural genomics
