1lkb
From Proteopedia
(New page: 200px<br /><applet load="1lkb" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lkb, resolution 1.70Å" /> '''Porcine Pancreatic E...) |
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| - | [[Image:1lkb.jpg|left|200px]]<br /><applet load="1lkb" size=" | + | [[Image:1lkb.jpg|left|200px]]<br /><applet load="1lkb" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1lkb, resolution 1.70Å" /> | caption="1lkb, resolution 1.70Å" /> | ||
'''Porcine Pancreatic Elastase/Na-Complex'''<br /> | '''Porcine Pancreatic Elastase/Na-Complex'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Porcine pancreatic elastase has been crystallized at slightly acidic pH | + | Porcine pancreatic elastase has been crystallized at slightly acidic pH under two similar but slightly different conditions. Diffraction data were collected at a wavelength of 1.5 A to a maximum resolution of 1.7 A. Both difference electron-density maps and anomalous difference electron-density maps suggest that in crystals grown from a sodium sulfate solution PPE binds Na(+) in its metal-binding site. In contrast, PPE binds Ca(2+) in crystals grown from a solution containing sodium citrate and calcium chloride. This observation is in contradiction to most PPE structures reported in the PDB. In addition to the metal-binding site, up to three other binding sites, which appear to be anion-binding sites, could be identified based on the observed anomalous intensity differences. |
==About this Structure== | ==About this Structure== | ||
| - | 1LKB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa] with SO4, CL and NA as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Pancreatic_elastase Pancreatic elastase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.36 3.4.21.36] Full crystallographic information is available from [http:// | + | 1LKB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa] with <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=CL:'>CL</scene> and <scene name='pdbligand=NA:'>NA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Pancreatic_elastase Pancreatic elastase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.36 3.4.21.36] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LKB OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Nowak, E.]] | [[Category: Nowak, E.]] | ||
[[Category: Panjikar, S.]] | [[Category: Panjikar, S.]] | ||
| - | [[Category: Tucker, P | + | [[Category: Tucker, P A.]] |
| - | [[Category: Weiss, M | + | [[Category: Weiss, M S.]] |
[[Category: CL]] | [[Category: CL]] | ||
[[Category: NA]] | [[Category: NA]] | ||
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[[Category: sulfate binding]] | [[Category: sulfate binding]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:45:43 2008'' |
Revision as of 11:45, 21 February 2008
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Porcine Pancreatic Elastase/Na-Complex
Overview
Porcine pancreatic elastase has been crystallized at slightly acidic pH under two similar but slightly different conditions. Diffraction data were collected at a wavelength of 1.5 A to a maximum resolution of 1.7 A. Both difference electron-density maps and anomalous difference electron-density maps suggest that in crystals grown from a sodium sulfate solution PPE binds Na(+) in its metal-binding site. In contrast, PPE binds Ca(2+) in crystals grown from a solution containing sodium citrate and calcium chloride. This observation is in contradiction to most PPE structures reported in the PDB. In addition to the metal-binding site, up to three other binding sites, which appear to be anion-binding sites, could be identified based on the observed anomalous intensity differences.
About this Structure
1LKB is a Single protein structure of sequence from Sus scrofa with , and as ligands. Active as Pancreatic elastase, with EC number 3.4.21.36 Full crystallographic information is available from OCA.
Reference
Metal binding to porcine pancreatic elastase: calcium or not calcium., Weiss MS, Panjikar S, Nowak E, Tucker PA, Acta Crystallogr D Biol Crystallogr. 2002 Sep;58(Pt 9):1407-12. Epub 2002, Aug 23. PMID:12198296
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