1lp3
From Proteopedia
(New page: 200px<br /><applet load="1lp3" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lp3, resolution 3.0Å" /> '''The Atomic Structure ...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1lp3.gif|left|200px]]<br /><applet load="1lp3" size=" | + | [[Image:1lp3.gif|left|200px]]<br /><applet load="1lp3" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1lp3, resolution 3.0Å" /> | caption="1lp3, resolution 3.0Å" /> | ||
'''The Atomic Structure of Adeno-Associated Virus (AAV-2), a Vector for Human Gene Therapy'''<br /> | '''The Atomic Structure of Adeno-Associated Virus (AAV-2), a Vector for Human Gene Therapy'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The structure of the adeno-associated virus (AAV-2) has been determined to | + | The structure of the adeno-associated virus (AAV-2) has been determined to 3-A resolution by x-ray crystallography. AAV is being developed as a vector for gene therapy to treat diseases including hemophilia, cancer, and cystic fibrosis. As in the distantly related autonomous parvoviruses, the capsid protein has a beta-barrel fold, but long loops between the beta-strands share little structural homology with other parvoviruses, leading to unique surface features. Most prominent are groups of threefold-related peaks, each an intimate association of loops from two neighboring subunits. Mutations affecting cell entry and receptor binding are clustered near the positively charged side of each peak, implicating the region in attachment to the cellular receptor, heparan sulfate proteoglycan. Amino acids involved in antibody binding are in the same general vicinity. The structure will guide rational engineering of vector capsids to tailor cellular targeting and to avoid immediate neutralization by an immune system sensitized by prior exposure to AAV. |
==About this Structure== | ==About this Structure== | ||
| - | 1LP3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Adeno-associated_virus_2 Adeno-associated virus 2]. Full crystallographic information is available from [http:// | + | 1LP3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Adeno-associated_virus_2 Adeno-associated virus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LP3 OCA]. |
==Reference== | ==Reference== | ||
| Line 16: | Line 16: | ||
[[Category: Bhatia, S.]] | [[Category: Bhatia, S.]] | ||
[[Category: Bu, W.]] | [[Category: Bu, W.]] | ||
| - | [[Category: Chapman, M | + | [[Category: Chapman, M S.]] |
[[Category: Hare, J.]] | [[Category: Hare, J.]] | ||
[[Category: Somasundaram, T.]] | [[Category: Somasundaram, T.]] | ||
| Line 29: | Line 29: | ||
[[Category: single-stranded]] | [[Category: single-stranded]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:47:02 2008'' |
Revision as of 11:47, 21 February 2008
|
The Atomic Structure of Adeno-Associated Virus (AAV-2), a Vector for Human Gene Therapy
Overview
The structure of the adeno-associated virus (AAV-2) has been determined to 3-A resolution by x-ray crystallography. AAV is being developed as a vector for gene therapy to treat diseases including hemophilia, cancer, and cystic fibrosis. As in the distantly related autonomous parvoviruses, the capsid protein has a beta-barrel fold, but long loops between the beta-strands share little structural homology with other parvoviruses, leading to unique surface features. Most prominent are groups of threefold-related peaks, each an intimate association of loops from two neighboring subunits. Mutations affecting cell entry and receptor binding are clustered near the positively charged side of each peak, implicating the region in attachment to the cellular receptor, heparan sulfate proteoglycan. Amino acids involved in antibody binding are in the same general vicinity. The structure will guide rational engineering of vector capsids to tailor cellular targeting and to avoid immediate neutralization by an immune system sensitized by prior exposure to AAV.
About this Structure
1LP3 is a Single protein structure of sequence from Adeno-associated virus 2. Full crystallographic information is available from OCA.
Reference
The atomic structure of adeno-associated virus (AAV-2), a vector for human gene therapy., Xie Q, Bu W, Bhatia S, Hare J, Somasundaram T, Azzi A, Chapman MS, Proc Natl Acad Sci U S A. 2002 Aug 6;99(16):10405-10. Epub 2002 Jul 22. PMID:12136130
Page seeded by OCA on Thu Feb 21 13:47:02 2008
Categories: Adeno-associated virus 2 | Single protein | Azzi, A. | Bhatia, S. | Bu, W. | Chapman, M S. | Hare, J. | Somasundaram, T. | Xie, Q. | Beta-barrel | Capsid | Dna | Human | Icosahedral virus | Parvovirus | Satellite | Single-stranded
