1lrr
From Proteopedia
(New page: 200px<br /><applet load="1lrr" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lrr, resolution 2.65Å" /> '''CRYSTAL STRUCTURE OF...) |
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| - | [[Image:1lrr.gif|left|200px]]<br /><applet load="1lrr" size=" | + | [[Image:1lrr.gif|left|200px]]<br /><applet load="1lrr" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1lrr, resolution 2.65Å" /> | caption="1lrr, resolution 2.65Å" /> | ||
'''CRYSTAL STRUCTURE OF E. COLI SEQA COMPLEXED WITH HEMIMETHYLATED DNA'''<br /> | '''CRYSTAL STRUCTURE OF E. COLI SEQA COMPLEXED WITH HEMIMETHYLATED DNA'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The SeqA protein binds clusters of fully methylated or hemimethylated GATC | + | The SeqA protein binds clusters of fully methylated or hemimethylated GATC sequences at oriC and negatively modulates the initiation of DNA replication. We find that SeqA can be proteolytically cleaved into an N-terminal multimerization and a C-terminal DNA-binding domain and have determined the crystal structure of the C-terminal domain in complex with a hemimethylated GATC site. SeqA makes direct hydrogen bonds and van der Waals contacts with the hemimethylated A-T base pair in addition to interactions with the surrounding bases and DNA backbone. The tetrameric protein-DNA complex found in the crystal suggests that SeqA binds multiple GATC sites on separate DNA duplexes, altering the overall DNA topology and sequestering oriC from replication initiation. |
==About this Structure== | ==About this Structure== | ||
| - | 1LRR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http:// | + | 1LRR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LRR OCA]. |
==Reference== | ==Reference== | ||
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[[Category: replication]] | [[Category: replication]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:47:51 2008'' |
Revision as of 11:47, 21 February 2008
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CRYSTAL STRUCTURE OF E. COLI SEQA COMPLEXED WITH HEMIMETHYLATED DNA
Overview
The SeqA protein binds clusters of fully methylated or hemimethylated GATC sequences at oriC and negatively modulates the initiation of DNA replication. We find that SeqA can be proteolytically cleaved into an N-terminal multimerization and a C-terminal DNA-binding domain and have determined the crystal structure of the C-terminal domain in complex with a hemimethylated GATC site. SeqA makes direct hydrogen bonds and van der Waals contacts with the hemimethylated A-T base pair in addition to interactions with the surrounding bases and DNA backbone. The tetrameric protein-DNA complex found in the crystal suggests that SeqA binds multiple GATC sites on separate DNA duplexes, altering the overall DNA topology and sequestering oriC from replication initiation.
About this Structure
1LRR is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Insights into negative modulation of E. coli replication initiation from the structure of SeqA-hemimethylated DNA complex., Guarne A, Zhao Q, Ghirlando R, Yang W, Nat Struct Biol. 2002 Nov;9(11):839-43. PMID:12379844
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