1ltx
From Proteopedia
(New page: 200px<br /><applet load="1ltx" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ltx, resolution 2.70Å" /> '''Structure of Rab Esc...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1ltx.gif|left|200px]]<br /><applet load="1ltx" size=" | + | [[Image:1ltx.gif|left|200px]]<br /><applet load="1ltx" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1ltx, resolution 2.70Å" /> | caption="1ltx, resolution 2.70Å" /> | ||
'''Structure of Rab Escort Protein-1 in complex with Rab geranylgeranyl transferase and isoprenoid'''<br /> | '''Structure of Rab Escort Protein-1 in complex with Rab geranylgeranyl transferase and isoprenoid'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Posttranslational geranylgeranylation of Rab GTPases is catalyzed by Rab | + | Posttranslational geranylgeranylation of Rab GTPases is catalyzed by Rab geranylgeranyltransferase (RabGGTase), which consists of a catalytic alpha/beta heterodimer and an accessory Rab escort protein (REP). The crystal structure of isoprenoid-bound RabGGTase complexed to REP-1 has been solved to 2.7 A resolution. The complex interface buries a surprisingly small surface area of ca. 680 A and is unexpectedly formed by helices 8, 10, and 12 of the RabGGTase alpha subunit and helices D and E of REP-1. We demonstrate that the affinity of RabGGTase for REP-1 is allosterically regulated by phosphoisoprenoid via a long-range trans-domain signal transduction event. Comparing the structure of REP-1 with the closely related RabGDI, we conclude that the specificity of the REP:RabGGTase interaction is defined by differently positioned phenylalanine residues conserved in the REP and GDI subfamilies. |
==About this Structure== | ==About this Structure== | ||
| - | 1LTX is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with ZN, CL and FAR as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1LTX is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with <scene name='pdbligand=ZN:'>ZN</scene>, <scene name='pdbligand=CL:'>CL</scene> and <scene name='pdbligand=FAR:'>FAR</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LTX OCA]. |
==Reference== | ==Reference== | ||
| Line 14: | Line 14: | ||
[[Category: Rattus norvegicus]] | [[Category: Rattus norvegicus]] | ||
[[Category: Alexandrov, K.]] | [[Category: Alexandrov, K.]] | ||
| - | [[Category: Goody, R | + | [[Category: Goody, R S.]] |
[[Category: Niculae, A.]] | [[Category: Niculae, A.]] | ||
[[Category: Pylypenko, O.]] | [[Category: Pylypenko, O.]] | ||
| Line 20: | Line 20: | ||
[[Category: Reents, R.]] | [[Category: Reents, R.]] | ||
[[Category: Schlichting, I.]] | [[Category: Schlichting, I.]] | ||
| - | [[Category: Thoma, N | + | [[Category: Thoma, N H.]] |
[[Category: Waldmann, H.]] | [[Category: Waldmann, H.]] | ||
[[Category: CL]] | [[Category: CL]] | ||
| Line 30: | Line 30: | ||
[[Category: rab prenylation]] | [[Category: rab prenylation]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:48:25 2008'' |
Revision as of 11:48, 21 February 2008
|
Structure of Rab Escort Protein-1 in complex with Rab geranylgeranyl transferase and isoprenoid
Overview
Posttranslational geranylgeranylation of Rab GTPases is catalyzed by Rab geranylgeranyltransferase (RabGGTase), which consists of a catalytic alpha/beta heterodimer and an accessory Rab escort protein (REP). The crystal structure of isoprenoid-bound RabGGTase complexed to REP-1 has been solved to 2.7 A resolution. The complex interface buries a surprisingly small surface area of ca. 680 A and is unexpectedly formed by helices 8, 10, and 12 of the RabGGTase alpha subunit and helices D and E of REP-1. We demonstrate that the affinity of RabGGTase for REP-1 is allosterically regulated by phosphoisoprenoid via a long-range trans-domain signal transduction event. Comparing the structure of REP-1 with the closely related RabGDI, we conclude that the specificity of the REP:RabGGTase interaction is defined by differently positioned phenylalanine residues conserved in the REP and GDI subfamilies.
About this Structure
1LTX is a Protein complex structure of sequences from Rattus norvegicus with , and as ligands. Full crystallographic information is available from OCA.
Reference
Structure of Rab escort protein-1 in complex with Rab geranylgeranyltransferase., Pylypenko O, Rak A, Reents R, Niculae A, Sidorovitch V, Cioaca MD, Bessolitsyna E, Thoma NH, Waldmann H, Schlichting I, Goody RS, Alexandrov K, Mol Cell. 2003 Feb;11(2):483-94. PMID:12620235
Page seeded by OCA on Thu Feb 21 13:48:25 2008
