1lx7
From Proteopedia
(New page: 200px<br /><applet load="1lx7" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lx7, resolution 2.00Å" /> '''Structure of E. coli...) |
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| - | [[Image:1lx7.gif|left|200px]]<br /><applet load="1lx7" size=" | + | [[Image:1lx7.gif|left|200px]]<br /><applet load="1lx7" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1lx7, resolution 2.00Å" /> | caption="1lx7, resolution 2.00Å" /> | ||
'''Structure of E. coli uridine phosphorylase at 2.0A'''<br /> | '''Structure of E. coli uridine phosphorylase at 2.0A'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The 2.0 A crystal structure has been determined for Escherichia coli | + | The 2.0 A crystal structure has been determined for Escherichia coli uridine phosphorylase (UP), an essential enzyme in nucleotide biosynthesis that catalyzes the phosphorolytic cleavage of the C-N glycosidic bond of uridine to ribose-1-phosphate and uracil. The structure determination of two independent monomers in the asymmetric unit revealed the residue composition and atomic details of the apo configurations of each active site. The native hexameric UP enzyme was revealed by applying threefold crystallographic symmetry to the contents of the asymmetric unit. The 2.0 A model reveals a closer structural relationship to other nucleotide phosphorylase enzymes than was previously appreciated. |
==About this Structure== | ==About this Structure== | ||
| - | 1LX7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Active as [http://en.wikipedia.org/wiki/Uridine_phosphorylase Uridine phosphorylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.3 2.4.2.3] Full crystallographic information is available from [http:// | + | 1LX7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Active as [http://en.wikipedia.org/wiki/Uridine_phosphorylase Uridine phosphorylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.3 2.4.2.3] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LX7 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Uridine phosphorylase]] | [[Category: Uridine phosphorylase]] | ||
[[Category: Beckwith, A.]] | [[Category: Beckwith, A.]] | ||
| - | [[Category: Buglino, J | + | [[Category: Buglino, J A.]] |
| - | [[Category: Burley, S | + | [[Category: Burley, S K.]] |
[[Category: Burling, T.]] | [[Category: Burling, T.]] | ||
[[Category: Chadna, T.]] | [[Category: Chadna, T.]] | ||
[[Category: Kniewel, R.]] | [[Category: Kniewel, R.]] | ||
| - | [[Category: Lima, C | + | [[Category: Lima, C D.]] |
| - | [[Category: NYSGXRC, New | + | [[Category: NYSGXRC, New York Structural GenomiX Research Consortium.]] |
[[Category: new york structural genomix research consortium]] | [[Category: new york structural genomix research consortium]] | ||
[[Category: nucleotide metabolism]] | [[Category: nucleotide metabolism]] | ||
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[[Category: udrpase]] | [[Category: udrpase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:49:20 2008'' |
Revision as of 11:49, 21 February 2008
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Structure of E. coli uridine phosphorylase at 2.0A
Overview
The 2.0 A crystal structure has been determined for Escherichia coli uridine phosphorylase (UP), an essential enzyme in nucleotide biosynthesis that catalyzes the phosphorolytic cleavage of the C-N glycosidic bond of uridine to ribose-1-phosphate and uracil. The structure determination of two independent monomers in the asymmetric unit revealed the residue composition and atomic details of the apo configurations of each active site. The native hexameric UP enzyme was revealed by applying threefold crystallographic symmetry to the contents of the asymmetric unit. The 2.0 A model reveals a closer structural relationship to other nucleotide phosphorylase enzymes than was previously appreciated.
About this Structure
1LX7 is a Single protein structure of sequence from Escherichia coli. Active as Uridine phosphorylase, with EC number 2.4.2.3 Full crystallographic information is available from OCA.
Reference
Structure of Escherichia coli uridine phosphorylase at 2.0 A., Burling FT, Kniewel R, Buglino JA, Chadha T, Beckwith A, Lima CD, Acta Crystallogr D Biol Crystallogr. 2003 Jan;59(Pt 1):73-6. Epub 2002 Dec, 19. PMID:12499542
Page seeded by OCA on Thu Feb 21 13:49:20 2008
Categories: Escherichia coli | Single protein | Uridine phosphorylase | Beckwith, A. | Buglino, J A. | Burley, S K. | Burling, T. | Chadna, T. | Kniewel, R. | Lima, C D. | NYSGXRC, New York Structural GenomiX Research Consortium. | New york structural genomix research consortium | Nucleotide metabolism | Nysgxrc | P12758 | Phosphorylase | Protein structure initiative | Psi | Structural genomics | Udrpase
