1gt6
From Proteopedia
(New page: 200px<br /> <applet load="1gt6" size="450" color="white" frame="true" align="right" spinBox="true" caption="1gt6, resolution 2.2Å" /> '''S146A MUTANT OF THER...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1GT6 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Thermomyces_lanuginosus Thermomyces lanuginosus]] with OLA as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GT6 OCA]]. | + | 1GT6 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Thermomyces_lanuginosus Thermomyces lanuginosus]] with OLA as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3]]. Structure known Active Site: OLA. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GT6 OCA]]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Thermomyces lanuginosus]] | [[Category: Thermomyces lanuginosus]] | ||
| + | [[Category: Triacylglycerol lipase]] | ||
[[Category: Brzozowski, A.M.]] | [[Category: Brzozowski, A.M.]] | ||
[[Category: Ivanova, M.G.]] | [[Category: Ivanova, M.G.]] | ||
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[[Category: zymogen]] | [[Category: zymogen]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 13:18:04 2007'' |
Revision as of 11:13, 30 October 2007
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S146A MUTANT OF THERMOMYCES (HUMICOLA) LANUGINOSA LIPASE COMPLEX WITH OLEIC ACID
Overview
The binding of Thermomyces lanuginosa lipase and its mutants [TLL(S146A), TLL(W89L), TLL(W117F, W221H, W260H)] to the mixed micelles of, cis-parinaric acid/sodium taurodeoxycholate at pH 5.0 led to the quenching, of the intrinsic tryptophan fluorescence emission (300-380 nm) and to a, simultaneous increase in the cis-parinaric acid fluorescence emission, (380-500 nm). These findings were used to characterize the Thermomyces, lanuginosa lipase/cis-parinaric acid interactions occurring in the, presence of sodium taurodeoxycholate.The fluorescence resonance energy, transfer and Stern-Volmer quenching constant values obtained were, correlated with the accessibility of the tryptophan residues to the, cis-parinaric acid and with the lid opening ability of Thermomyces, lanuginosa lipase (and its ... [(full description)]
About this Structure
1GT6 is a [Single protein] structure of sequence from [Thermomyces lanuginosus] with OLA as [ligand]. Active as [Triacylglycerol lipase], with EC number [3.1.1.3]. Structure known Active Site: OLA. Full crystallographic information is available from [OCA].
Reference
Binding of Thermomyces (Humicola) lanuginosa lipase to the mixed micelles of cis-parinaric acid/NaTDC., Yapoudjian S, Ivanova MG, Brzozowski AM, Patkar SA, Vind J, Svendsen A, Verger R, Eur J Biochem. 2002 Mar;269(6):1613-21. PMID:11895431
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