1ly1
From Proteopedia
(New page: 200px<br /><applet load="1ly1" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ly1, resolution 2.00Å" /> '''Structure and Mechan...) |
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| - | [[Image:1ly1.jpg|left|200px]]<br /><applet load="1ly1" size=" | + | [[Image:1ly1.jpg|left|200px]]<br /><applet load="1ly1" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1ly1, resolution 2.00Å" /> | caption="1ly1, resolution 2.00Å" /> | ||
'''Structure and Mechanism of T4 Polynucleotide Kinase'''<br /> | '''Structure and Mechanism of T4 Polynucleotide Kinase'''<br /> | ||
==Overview== | ==Overview== | ||
| - | T4 polynucleotide kinase (Pnk), in addition to being an invaluable | + | T4 polynucleotide kinase (Pnk), in addition to being an invaluable research tool, exemplifies a family of bifunctional enzymes with 5'-kinase and 3'-phosphatase activities that play key roles in RNA and DNA repair. T4 Pnk is a homotetramer composed of a C-terminal phosphatase domain and an N-terminal kinase domain. The 2.0 A crystal structure of the isolated kinase domain highlights a tunnel-like active site through the heart of the enzyme, with an entrance on the 5' OH acceptor side that can accommodate a single-stranded polynucleotide. The active site is composed of essential side chains that coordinate the beta phosphate of the NTP donor and the 3' phosphate of the 5' OH acceptor, plus a putative general acid that activates the 5' OH. The structure rationalizes the different specificities of T4 and eukaryotic Pnk and suggests a model for the assembly of the tetramer. |
==About this Structure== | ==About this Structure== | ||
| - | 1LY1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t4 Bacteriophage t4] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Polynucleotide_5'-hydroxy-kinase Polynucleotide 5'-hydroxy-kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.78 2.7.1.78] Full crystallographic information is available from [http:// | + | 1LY1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t4 Bacteriophage t4] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Polynucleotide_5'-hydroxy-kinase Polynucleotide 5'-hydroxy-kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.78 2.7.1.78] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LY1 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Polynucleotide 5'-hydroxy-kinase]] | [[Category: Polynucleotide 5'-hydroxy-kinase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Lima, C | + | [[Category: Lima, C D.]] |
[[Category: Shuman, S.]] | [[Category: Shuman, S.]] | ||
| - | [[Category: Wang, L | + | [[Category: Wang, L K.]] |
[[Category: SO4]] | [[Category: SO4]] | ||
[[Category: kinase]] | [[Category: kinase]] | ||
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[[Category: t4]] | [[Category: t4]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:49:28 2008'' |
Revision as of 11:49, 21 February 2008
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Structure and Mechanism of T4 Polynucleotide Kinase
Overview
T4 polynucleotide kinase (Pnk), in addition to being an invaluable research tool, exemplifies a family of bifunctional enzymes with 5'-kinase and 3'-phosphatase activities that play key roles in RNA and DNA repair. T4 Pnk is a homotetramer composed of a C-terminal phosphatase domain and an N-terminal kinase domain. The 2.0 A crystal structure of the isolated kinase domain highlights a tunnel-like active site through the heart of the enzyme, with an entrance on the 5' OH acceptor side that can accommodate a single-stranded polynucleotide. The active site is composed of essential side chains that coordinate the beta phosphate of the NTP donor and the 3' phosphate of the 5' OH acceptor, plus a putative general acid that activates the 5' OH. The structure rationalizes the different specificities of T4 and eukaryotic Pnk and suggests a model for the assembly of the tetramer.
About this Structure
1LY1 is a Single protein structure of sequence from Bacteriophage t4 with as ligand. Active as Polynucleotide 5'-hydroxy-kinase, with EC number 2.7.1.78 Full crystallographic information is available from OCA.
Reference
Structure and mechanism of T4 polynucleotide kinase: an RNA repair enzyme., Wang LK, Lima CD, Shuman S, EMBO J. 2002 Jul 15;21(14):3873-80. PMID:12110598
Page seeded by OCA on Thu Feb 21 13:49:28 2008
Categories: Bacteriophage t4 | Polynucleotide 5'-hydroxy-kinase | Single protein | Lima, C D. | Shuman, S. | Wang, L K. | SO4 | Kinase | Phage | Phosphatase | Pnk | Polynucleotide | T4
