1m2t

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(New page: 200px<br /><applet load="1m2t" size="450" color="white" frame="true" align="right" spinBox="true" caption="1m2t, resolution 1.89&Aring;" /> '''Mistletoe Lectin I f...)
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[[Image:1m2t.gif|left|200px]]<br /><applet load="1m2t" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1m2t, resolution 1.89&Aring;" />
caption="1m2t, resolution 1.89&Aring;" />
'''Mistletoe Lectin I from Viscum album in Complex with Adenine Monophosphate. Crystal Structure at 1.9 A Resolution'''<br />
'''Mistletoe Lectin I from Viscum album in Complex with Adenine Monophosphate. Crystal Structure at 1.9 A Resolution'''<br />
==Overview==
==Overview==
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The crystal structure of the ribosome-inactivating protein (RIP) mistletoe, lectin I (ML-I) from Viscum album in complex with adenine has been refined, to 1.9 A resolution. High quality crystals of the ML-I complex were, obtained by the method of vapour diffusion using the high density protein, crystal growth system (HDPCG) on the international space station, mission, ISS 6A. Hexagonal crystals were grown during three months under, microgravity conditions. Diffraction data to 1.9A were collected applying, synchrotron radiation and cryo- techniques. The structure was refined, subsequently to analyse the structure of ML-I and particularly the active, site conformation, complexed by adenine that mimics the RNA substrate, binding.
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The crystal structure of the ribosome-inactivating protein (RIP) mistletoe lectin I (ML-I) from Viscum album in complex with adenine has been refined to 1.9 A resolution. High quality crystals of the ML-I complex were obtained by the method of vapour diffusion using the high density protein crystal growth system (HDPCG) on the international space station, mission ISS 6A. Hexagonal crystals were grown during three months under microgravity conditions. Diffraction data to 1.9A were collected applying synchrotron radiation and cryo- techniques. The structure was refined subsequently to analyse the structure of ML-I and particularly the active site conformation, complexed by adenine that mimics the RNA substrate binding.
==About this Structure==
==About this Structure==
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1M2T is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Viscum_album Viscum album] with NAG, FUC, ADE and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1M2T OCA].
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1M2T is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Viscum_album Viscum album] with <scene name='pdbligand=NAG:'>NAG</scene>, <scene name='pdbligand=FUC:'>FUC</scene>, <scene name='pdbligand=ADE:'>ADE</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M2T OCA].
==Reference==
==Reference==
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[[Category: ribosome inactivation]]
[[Category: ribosome inactivation]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 21:06:07 2007''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:50:54 2008''

Revision as of 11:50, 21 February 2008

Image:1m2t.gif

1m2t, resolution 1.89Å

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Mistletoe Lectin I from Viscum album in Complex with Adenine Monophosphate. Crystal Structure at 1.9 A Resolution

Overview

The crystal structure of the ribosome-inactivating protein (RIP) mistletoe lectin I (ML-I) from Viscum album in complex with adenine has been refined to 1.9 A resolution. High quality crystals of the ML-I complex were obtained by the method of vapour diffusion using the high density protein crystal growth system (HDPCG) on the international space station, mission ISS 6A. Hexagonal crystals were grown during three months under microgravity conditions. Diffraction data to 1.9A were collected applying synchrotron radiation and cryo- techniques. The structure was refined subsequently to analyse the structure of ML-I and particularly the active site conformation, complexed by adenine that mimics the RNA substrate binding.

About this Structure

1M2T is a Protein complex structure of sequences from Viscum album with , , and as ligands. Active as rRNA N-glycosylase, with EC number 3.2.2.22 Full crystallographic information is available from OCA.

Reference

Crystallisation under microgravity of mistletoe lectin I from Viscum album with adenine monophosphate and the crystal structure at 1.9 A resolution., Krauspenhaar R, Rypniewski W, Kalkura N, Moore K, DeLucas L, Stoeva S, Mikhailov A, Voelter W, Betzel Ch, Acta Crystallogr D Biol Crystallogr. 2002 Oct;58(Pt 10 Pt 1):1704-7. Epub, 2002 Sep 26. PMID:12351890

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