1mli
From Proteopedia
(New page: 200px<br /><applet load="1mli" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mli, resolution 3.3Å" /> '''CRYSTAL STRUCTURE OF ...) |
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| - | [[Image:1mli.gif|left|200px]]<br /><applet load="1mli" size=" | + | [[Image:1mli.gif|left|200px]]<br /><applet load="1mli" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1mli, resolution 3.3Å" /> | caption="1mli, resolution 3.3Å" /> | ||
'''CRYSTAL STRUCTURE OF MUCONOLACTONE ISOMERASE AT 3.3 ANGSTROMS RESOLUTION'''<br /> | '''CRYSTAL STRUCTURE OF MUCONOLACTONE ISOMERASE AT 3.3 ANGSTROMS RESOLUTION'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystal structure of muconolactone isomerase from Pseudomonas putida, a unique molecule with ten 96 amino acid subunits and 5-fold, and 2-fold | + | The crystal structure of muconolactone isomerase from Pseudomonas putida, a unique molecule with ten 96 amino acid subunits and 5-fold, and 2-fold symmetries, has been solved at 3.3 A resolution. The non-crystallographic symmetries were used to refine the initial single isomorphous replacement phases and produce an interpretable 10-fold averaged map. The backbone trace is complete and confirmed by the amino acid sequence fit. Each subunit is composed of a body with two alpha-helices and an antiparallel twisted beta-sheet of four strands, and an extended arm. The helices and the sheet fold to form a two-layered structure with an enclosed hydrophobic core and a partially formed putative active site pocket. The C-terminal arm of another subunit related by a local dyad symmetry extends over the core to complete this pocket. The decameric protein is almost spherical, with the helices forming the external coat. There is a large hydrophilic cavity in the center with open ends along the 5-fold axis. Molecular interactions between subunits are extensive. Each subunit contacts four neighbors and loses nearly 40% of its solvent contact area on oligomerization. |
==About this Structure== | ==About this Structure== | ||
| - | 1MLI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Active as [http://en.wikipedia.org/wiki/Muconolactone_Delta-isomerase Muconolactone Delta-isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.3.4 5.3.3.4] Full crystallographic information is available from [http:// | + | 1MLI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Active as [http://en.wikipedia.org/wiki/Muconolactone_Delta-isomerase Muconolactone Delta-isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.3.4 5.3.3.4] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MLI OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Pseudomonas putida]] | [[Category: Pseudomonas putida]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Katti, S | + | [[Category: Katti, S K.]] |
| - | [[Category: Katz, B | + | [[Category: Katz, B A.]] |
| - | [[Category: Wyckoff, H | + | [[Category: Wyckoff, H W.]] |
[[Category: intramolecular oxidoreductase]] | [[Category: intramolecular oxidoreductase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:56:24 2008'' |
Revision as of 11:56, 21 February 2008
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CRYSTAL STRUCTURE OF MUCONOLACTONE ISOMERASE AT 3.3 ANGSTROMS RESOLUTION
Overview
The crystal structure of muconolactone isomerase from Pseudomonas putida, a unique molecule with ten 96 amino acid subunits and 5-fold, and 2-fold symmetries, has been solved at 3.3 A resolution. The non-crystallographic symmetries were used to refine the initial single isomorphous replacement phases and produce an interpretable 10-fold averaged map. The backbone trace is complete and confirmed by the amino acid sequence fit. Each subunit is composed of a body with two alpha-helices and an antiparallel twisted beta-sheet of four strands, and an extended arm. The helices and the sheet fold to form a two-layered structure with an enclosed hydrophobic core and a partially formed putative active site pocket. The C-terminal arm of another subunit related by a local dyad symmetry extends over the core to complete this pocket. The decameric protein is almost spherical, with the helices forming the external coat. There is a large hydrophilic cavity in the center with open ends along the 5-fold axis. Molecular interactions between subunits are extensive. Each subunit contacts four neighbors and loses nearly 40% of its solvent contact area on oligomerization.
About this Structure
1MLI is a Single protein structure of sequence from Pseudomonas putida. Active as Muconolactone Delta-isomerase, with EC number 5.3.3.4 Full crystallographic information is available from OCA.
Reference
Crystal structure of muconolactone isomerase at 3.3 A resolution., Katti SK, Katz BA, Wyckoff HW, J Mol Biol. 1989 Feb 5;205(3):557-71. PMID:2926818
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