1mzb
From Proteopedia
(New page: 200px<br /><applet load="1mzb" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mzb, resolution 1.8Å" /> '''Ferric uptake regulat...) |
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| - | [[Image:1mzb.jpg|left|200px]]<br /><applet load="1mzb" size=" | + | [[Image:1mzb.jpg|left|200px]]<br /><applet load="1mzb" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1mzb, resolution 1.8Å" /> | caption="1mzb, resolution 1.8Å" /> | ||
'''Ferric uptake regulator'''<br /> | '''Ferric uptake regulator'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Iron is an essential element for almost all organisms, although an | + | Iron is an essential element for almost all organisms, although an overload of this element results in toxicity because of the formation of hydroxyl radicals. Consequently, most living entities have developed sophisticated mechanisms to control their intracellular iron concentration. In many bacteria, including the opportunistic pathogen Pseudomonas aeruginosa, this task is performed by the ferric uptake regulator (Fur). Fur controls a wide variety of basic physiological processes including iron uptake systems and the expression of exotoxin A. Here, we present the first crystal structure of Fur from P. aeruginosa in complex with Zn2+ determined at a resolution of 1.8 A. Furthermore, X-ray absorption spectroscopic measurements and microPIXE analysis were performed in order to characterize the distinct zinc and iron binding sites in solution. The combination of these complementary techniques enables us to present a model for the activation and DNA binding of the Fur protein. |
==About this Structure== | ==About this Structure== | ||
| - | 1MZB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1MZB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MZB OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Pseudomonas aeruginosa]] | [[Category: Pseudomonas aeruginosa]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Haller, J | + | [[Category: Haller, J C.]] |
[[Category: Pohl, E.]] | [[Category: Pohl, E.]] | ||
| - | [[Category: Vasil, M | + | [[Category: Vasil, M L.]] |
[[Category: ZN]] | [[Category: ZN]] | ||
[[Category: dtxr]] | [[Category: dtxr]] | ||
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[[Category: iron]] | [[Category: iron]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:00:32 2008'' |
Revision as of 12:00, 21 February 2008
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Ferric uptake regulator
Overview
Iron is an essential element for almost all organisms, although an overload of this element results in toxicity because of the formation of hydroxyl radicals. Consequently, most living entities have developed sophisticated mechanisms to control their intracellular iron concentration. In many bacteria, including the opportunistic pathogen Pseudomonas aeruginosa, this task is performed by the ferric uptake regulator (Fur). Fur controls a wide variety of basic physiological processes including iron uptake systems and the expression of exotoxin A. Here, we present the first crystal structure of Fur from P. aeruginosa in complex with Zn2+ determined at a resolution of 1.8 A. Furthermore, X-ray absorption spectroscopic measurements and microPIXE analysis were performed in order to characterize the distinct zinc and iron binding sites in solution. The combination of these complementary techniques enables us to present a model for the activation and DNA binding of the Fur protein.
About this Structure
1MZB is a Single protein structure of sequence from Pseudomonas aeruginosa with as ligand. Full crystallographic information is available from OCA.
Reference
Architecture of a protein central to iron homeostasis: crystal structure and spectroscopic analysis of the ferric uptake regulator., Pohl E, Haller JC, Mijovilovich A, Meyer-Klaucke W, Garman E, Vasil ML, Mol Microbiol. 2003 Feb;47(4):903-15. PMID:12581348
Page seeded by OCA on Thu Feb 21 14:00:32 2008
