1mzp
From Proteopedia
(New page: 200px<br /><applet load="1mzp" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mzp, resolution 2.65Å" /> '''Structure of the L1 ...) |
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| - | [[Image:1mzp.gif|left|200px]]<br /><applet load="1mzp" size=" | + | [[Image:1mzp.gif|left|200px]]<br /><applet load="1mzp" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1mzp, resolution 2.65Å" /> | caption="1mzp, resolution 2.65Å" /> | ||
'''Structure of the L1 protuberance in the ribosome'''<br /> | '''Structure of the L1 protuberance in the ribosome'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The L1 protuberance of the 50S ribosomal subunit is implicated in the | + | The L1 protuberance of the 50S ribosomal subunit is implicated in the release/disposal of deacylated tRNA from the E site. The apparent mobility of this ribosomal region has thus far prevented an accurate determination of its three-dimensional structure within either the 50S subunit or the 70S ribosome. Here we report the crystal structure at 2.65 A resolution of ribosomal protein L1 from Sulfolobus acidocaldarius in complex with a specific 55-nucleotide fragment of 23S rRNA from Thermus thermophilus. This structure fills a major gap in current models of the 50S ribosomal subunit. The conformations of L1 and of the rRNA fragment differ dramatically from those within the crystallographic model of the T. thermophilus 70S ribosome. Incorporation of the L1-rRNA complex into the structural models of the T. thermophilus 70S ribosome and the Deinococcus radiodurans 50S subunit gives a reliable representation of most of the L1 protuberance within the ribosome. |
==About this Structure== | ==About this Structure== | ||
| - | 1MZP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_acidocaldarius Sulfolobus acidocaldarius] with MG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1MZP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_acidocaldarius Sulfolobus acidocaldarius] with <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MZP OCA]. |
==Reference== | ==Reference== | ||
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[[Category: rna-protein complex]] | [[Category: rna-protein complex]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:00:40 2008'' |
Revision as of 12:00, 21 February 2008
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Structure of the L1 protuberance in the ribosome
Overview
The L1 protuberance of the 50S ribosomal subunit is implicated in the release/disposal of deacylated tRNA from the E site. The apparent mobility of this ribosomal region has thus far prevented an accurate determination of its three-dimensional structure within either the 50S subunit or the 70S ribosome. Here we report the crystal structure at 2.65 A resolution of ribosomal protein L1 from Sulfolobus acidocaldarius in complex with a specific 55-nucleotide fragment of 23S rRNA from Thermus thermophilus. This structure fills a major gap in current models of the 50S ribosomal subunit. The conformations of L1 and of the rRNA fragment differ dramatically from those within the crystallographic model of the T. thermophilus 70S ribosome. Incorporation of the L1-rRNA complex into the structural models of the T. thermophilus 70S ribosome and the Deinococcus radiodurans 50S subunit gives a reliable representation of most of the L1 protuberance within the ribosome.
About this Structure
1MZP is a Single protein structure of sequence from Sulfolobus acidocaldarius with as ligand. Full crystallographic information is available from OCA.
Reference
Structure of the L1 protuberance in the ribosome., Nikulin A, Eliseikina I, Tishchenko S, Nevskaya N, Davydova N, Platonova O, Piendl W, Selmer M, Liljas A, Drygin D, Zimmermann R, Garber M, Nikonov S, Nat Struct Biol. 2003 Feb;10(2):104-8. PMID:12514741
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