1n38
From Proteopedia
(New page: 200px<br /><applet load="1n38" size="450" color="white" frame="true" align="right" spinBox="true" caption="1n38, resolution 2.8Å" /> '''reovirus polymerase l...) |
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| - | [[Image:1n38.gif|left|200px]]<br /><applet load="1n38" size=" | + | [[Image:1n38.gif|left|200px]]<br /><applet load="1n38" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1n38, resolution 2.8Å" /> | caption="1n38, resolution 2.8Å" /> | ||
'''reovirus polymerase lambda3 elongation complex with one phosphodiester bond formed'''<br /> | '''reovirus polymerase lambda3 elongation complex with one phosphodiester bond formed'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The reovirus polymerase and those of other dsRNA viruses function within | + | The reovirus polymerase and those of other dsRNA viruses function within the confines of a protein capsid to transcribe the tightly packed dsRNA genome segments. The crystal structure of the reovirus polymerase, lambda3, determined at 2.5 A resolution, shows a fingers-palm-thumb core, similar to those of other viral polymerases, surrounded by major N- and C-terminal elaborations, which create a cage-like structure, with four channels leading to the catalytic site. This "caged" polymerase has allowed us to visualize the results of several rounds of RNA polymerization directly in the crystals. A 5' cap binding site on the surface of lambda3 suggests a template retention mechanism by which attachment of the 5' end of the plus-sense strand facilitates insertion of the 3' end of the minus-sense strand into the template channel. |
==About this Structure== | ==About this Structure== | ||
| - | 1N38 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mammalian_orthoreovirus_3 Mammalian orthoreovirus 3] with MN, U3H and CH1 as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1N38 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mammalian_orthoreovirus_3 Mammalian orthoreovirus 3] with <scene name='pdbligand=MN:'>MN</scene>, <scene name='pdbligand=U3H:'>U3H</scene> and <scene name='pdbligand=CH1:'>CH1</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N38 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Mammalian orthoreovirus 3]] | [[Category: Mammalian orthoreovirus 3]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Farsetta, D | + | [[Category: Farsetta, D L.]] |
| - | [[Category: Harrison, S | + | [[Category: Harrison, S C.]] |
| - | [[Category: Nibert, M | + | [[Category: Nibert, M L.]] |
[[Category: Tao, Y.]] | [[Category: Tao, Y.]] | ||
[[Category: CH1]] | [[Category: CH1]] | ||
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[[Category: rna polymerase]] | [[Category: rna polymerase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:01:45 2008'' |
Revision as of 12:01, 21 February 2008
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reovirus polymerase lambda3 elongation complex with one phosphodiester bond formed
Overview
The reovirus polymerase and those of other dsRNA viruses function within the confines of a protein capsid to transcribe the tightly packed dsRNA genome segments. The crystal structure of the reovirus polymerase, lambda3, determined at 2.5 A resolution, shows a fingers-palm-thumb core, similar to those of other viral polymerases, surrounded by major N- and C-terminal elaborations, which create a cage-like structure, with four channels leading to the catalytic site. This "caged" polymerase has allowed us to visualize the results of several rounds of RNA polymerization directly in the crystals. A 5' cap binding site on the surface of lambda3 suggests a template retention mechanism by which attachment of the 5' end of the plus-sense strand facilitates insertion of the 3' end of the minus-sense strand into the template channel.
About this Structure
1N38 is a Single protein structure of sequence from Mammalian orthoreovirus 3 with , and as ligands. Full crystallographic information is available from OCA.
Reference
RNA synthesis in a cage--structural studies of reovirus polymerase lambda3., Tao Y, Farsetta DL, Nibert ML, Harrison SC, Cell. 2002 Nov 27;111(5):733-45. PMID:12464184
Page seeded by OCA on Thu Feb 21 14:01:45 2008
