1nfv
From Proteopedia
(New page: 200px<br /><applet load="1nfv" size="450" color="white" frame="true" align="right" spinBox="true" caption="1nfv, resolution 1.95Å" /> '''X-ray structure of D...) |
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| - | [[Image:1nfv.gif|left|200px]]<br /><applet load="1nfv" size=" | + | [[Image:1nfv.gif|left|200px]]<br /><applet load="1nfv" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1nfv, resolution 1.95Å" /> | caption="1nfv, resolution 1.95Å" /> | ||
'''X-ray structure of Desulfovibrio desulfuricans bacterioferritin: the diiron centre in different catalytic states (as-isolated structure)'''<br /> | '''X-ray structure of Desulfovibrio desulfuricans bacterioferritin: the diiron centre in different catalytic states (as-isolated structure)'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The first crystal structure of a native di-iron center in an iron-storage | + | The first crystal structure of a native di-iron center in an iron-storage protein (bacterio)ferritin is reported. The protein, isolated from the anaerobic bacterium Desulfovibrio desulfuricans, has the unique property of having Fe-coproporphyrin III as its heme cofactor. The three-dimensional structure of this bacterioferritin was determined in three distinct catalytic/redox states by X-ray crystallography (at 1.95, 2.05 and 2.35 A resolution), corresponding to different intermediates of the di-iron ferroxidase site. Conformational changes associated with these intermediates support the idea of a route for iron entry into the protein shell through a pore that passes through the di-iron center. Molecular surface and electrostatic potential calculations also suggest the presence of another ion channel, distant from the channels at the three- and four-fold axes proposed as points of entry for the iron atoms. |
==About this Structure== | ==About this Structure== | ||
| - | 1NFV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Desulfovibrio_desulfuricans Desulfovibrio desulfuricans] with FE, SO4, 3PY, FEC and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1NFV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Desulfovibrio_desulfuricans Desulfovibrio desulfuricans] with <scene name='pdbligand=FE:'>FE</scene>, <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=3PY:'>3PY</scene>, <scene name='pdbligand=FEC:'>FEC</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NFV OCA]. |
==Reference== | ==Reference== | ||
| Line 13: | Line 13: | ||
[[Category: Desulfovibrio desulfuricans]] | [[Category: Desulfovibrio desulfuricans]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Carrondo, M | + | [[Category: Carrondo, M A.]] |
[[Category: LeGall, J.]] | [[Category: LeGall, J.]] | ||
[[Category: Lindley, P.]] | [[Category: Lindley, P.]] | ||
| - | [[Category: Liu, M | + | [[Category: Liu, M Y.]] |
[[Category: Macedo, S.]] | [[Category: Macedo, S.]] | ||
| - | [[Category: Matias, P | + | [[Category: Matias, P M.]] |
[[Category: Mitchell, E.]] | [[Category: Mitchell, E.]] | ||
| - | [[Category: Romao, C | + | [[Category: Romao, C V.]] |
[[Category: Teixeira, M.]] | [[Category: Teixeira, M.]] | ||
| - | [[Category: Xavier, A | + | [[Category: Xavier, A V.]] |
[[Category: 3PY]] | [[Category: 3PY]] | ||
[[Category: FE]] | [[Category: FE]] | ||
| Line 33: | Line 33: | ||
[[Category: haem fe-coproporphyrin iii cofactor]] | [[Category: haem fe-coproporphyrin iii cofactor]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:05:35 2008'' |
Revision as of 12:05, 21 February 2008
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X-ray structure of Desulfovibrio desulfuricans bacterioferritin: the diiron centre in different catalytic states (as-isolated structure)
Overview
The first crystal structure of a native di-iron center in an iron-storage protein (bacterio)ferritin is reported. The protein, isolated from the anaerobic bacterium Desulfovibrio desulfuricans, has the unique property of having Fe-coproporphyrin III as its heme cofactor. The three-dimensional structure of this bacterioferritin was determined in three distinct catalytic/redox states by X-ray crystallography (at 1.95, 2.05 and 2.35 A resolution), corresponding to different intermediates of the di-iron ferroxidase site. Conformational changes associated with these intermediates support the idea of a route for iron entry into the protein shell through a pore that passes through the di-iron center. Molecular surface and electrostatic potential calculations also suggest the presence of another ion channel, distant from the channels at the three- and four-fold axes proposed as points of entry for the iron atoms.
About this Structure
1NFV is a Single protein structure of sequence from Desulfovibrio desulfuricans with , , , and as ligands. Full crystallographic information is available from OCA.
Reference
The nature of the di-iron site in the bacterioferritin from Desulfovibrio desulfuricans., Macedo S, Romao CV, Mitchell E, Matias PM, Liu MY, Xavier AV, LeGall J, Teixeira M, Lindley P, Carrondo MA, Nat Struct Biol. 2003 Apr;10(4):285-90. PMID:12627224
Page seeded by OCA on Thu Feb 21 14:05:35 2008
Categories: Desulfovibrio desulfuricans | Single protein | Carrondo, M A. | LeGall, J. | Lindley, P. | Liu, M Y. | Macedo, S. | Matias, P M. | Mitchell, E. | Romao, C V. | Teixeira, M. | Xavier, A V. | 3PY | FE | FEC | GOL | SO4 | 24 subunits in the active molecule | Bacterioferritin | Diiron centre | Haem fe-coproporphyrin iii cofactor
