1nor
From Proteopedia
(New page: 200px<br /><applet load="1nor" size="450" color="white" frame="true" align="right" spinBox="true" caption="1nor" /> '''TWO-DIMENSIONAL 1H-NMR STUDY OF THE SPATIAL ...) |
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'''TWO-DIMENSIONAL 1H-NMR STUDY OF THE SPATIAL STRUCTURE OF NEUROTOXIN II FROM NAJA OXIANA'''<br /> | '''TWO-DIMENSIONAL 1H-NMR STUDY OF THE SPATIAL STRUCTURE OF NEUROTOXIN II FROM NAJA OXIANA'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The spatial structure of neurotoxin II from the venom of the central Asian | + | The spatial structure of neurotoxin II from the venom of the central Asian cobra Naja naja oxiana was determined by two-dimensional 1H-NMR techniques and computational analysis. Nearly complete proton resonance assignments for 61 amino acid residues have been made using two-dimensional (2D) homonuclear total correlated spectroscopy, 2D homonuclear double-quantum-filtered correlated spectroscopy and 2D homonuclear NOE spectroscopy (NOESY) experiments. The cross-peak volumes in NOESY spectra spin-spin coupling constants of vicinal protons NH-C alpha H and C alpha H-C beta H and the observation of slow deuterium exchange of amide protons were used to define local structure and a set of constraints for distance geometry program DIANA. The average root-mean-square deviations are 53 pm for backbone heavy atoms and 118 pm for all heavy atoms of 19 final neurotoxin II conformations. The spatial structure is characterized by a short double-stranded (residues 1-5 and 13-17) and a triple-stranded (residues 22-30, 33-41 and 50-54) antiparallel beta-sheets. |
==About this Structure== | ==About this Structure== | ||
| - | 1NOR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Naja_oxiana Naja oxiana]. Full crystallographic information is available from [http:// | + | 1NOR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Naja_oxiana Naja oxiana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NOR OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Naja oxiana]] | [[Category: Naja oxiana]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Arseniev, A | + | [[Category: Arseniev, A S.]] |
| - | [[Category: Golovanov, A | + | [[Category: Golovanov, A P.]] |
| - | [[Category: Lomize, A | + | [[Category: Lomize, A L.]] |
| - | [[Category: Tsetlin, V | + | [[Category: Tsetlin, V I.]] |
| - | [[Category: Utkin, Y | + | [[Category: Utkin, Y N.]] |
[[Category: neurotoxin]] | [[Category: neurotoxin]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:08:21 2008'' |
Revision as of 12:08, 21 February 2008
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TWO-DIMENSIONAL 1H-NMR STUDY OF THE SPATIAL STRUCTURE OF NEUROTOXIN II FROM NAJA OXIANA
Overview
The spatial structure of neurotoxin II from the venom of the central Asian cobra Naja naja oxiana was determined by two-dimensional 1H-NMR techniques and computational analysis. Nearly complete proton resonance assignments for 61 amino acid residues have been made using two-dimensional (2D) homonuclear total correlated spectroscopy, 2D homonuclear double-quantum-filtered correlated spectroscopy and 2D homonuclear NOE spectroscopy (NOESY) experiments. The cross-peak volumes in NOESY spectra spin-spin coupling constants of vicinal protons NH-C alpha H and C alpha H-C beta H and the observation of slow deuterium exchange of amide protons were used to define local structure and a set of constraints for distance geometry program DIANA. The average root-mean-square deviations are 53 pm for backbone heavy atoms and 118 pm for all heavy atoms of 19 final neurotoxin II conformations. The spatial structure is characterized by a short double-stranded (residues 1-5 and 13-17) and a triple-stranded (residues 22-30, 33-41 and 50-54) antiparallel beta-sheets.
About this Structure
1NOR is a Single protein structure of sequence from Naja oxiana. Full crystallographic information is available from OCA.
Reference
Two-dimensional 1H-NMR study of the spatial structure of neurotoxin II from Naja naja oxiana., Golovanov AP, Lomize AL, Arseniev AS, Utkin YN, Tsetlin VI, Eur J Biochem. 1993 May 1;213(3):1213-23. PMID:8504813
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