1nvm
From Proteopedia
(New page: 200px<br /><applet load="1nvm" size="450" color="white" frame="true" align="right" spinBox="true" caption="1nvm, resolution 1.70Å" /> '''Crystal structure of...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1nvm.jpg|left|200px]]<br /><applet load="1nvm" size=" | + | [[Image:1nvm.jpg|left|200px]]<br /><applet load="1nvm" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1nvm, resolution 1.70Å" /> | caption="1nvm, resolution 1.70Å" /> | ||
'''Crystal structure of a bifunctional aldolase-dehydrogenase : sequestering a reactive and volatile intermediate'''<br /> | '''Crystal structure of a bifunctional aldolase-dehydrogenase : sequestering a reactive and volatile intermediate'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystal structure of the bifunctional enzyme 4-hydroxy-2-ketovalerate | + | The crystal structure of the bifunctional enzyme 4-hydroxy-2-ketovalerate aldolase (DmpG)/acylating acetaldehyde dehydrogenase (DmpF), which is involved in the bacterial degradation of toxic aromatic compounds, has been determined by multiwavelength anomalous dispersion (MAD) techniques and refined to 1.7-A resolution. Structures of the two polypeptides represent a previously unrecognized subclass of metal-dependent aldolases, and of a CoA-dependent dehydrogenase. The structure reveals a mixed state of NAD+ binding to the DmpF protomer. Domain movements associated with cofactor binding in the DmpF protomer may be correlated with channeling and activity at the DmpG protomer. In the presence of NAD+ a 29-A-long sequestered tunnel links the two active sites. Two barriers are visible along the tunnel and suggest control points for the movement of the reactive and volatile acetaldehyde intermediate between the two active sites. |
==About this Structure== | ==About this Structure== | ||
| - | 1NVM is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Pseudomonas_sp. Pseudomonas sp.] with MN, OXL, SO4, NAD and MPD as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Acetaldehyde_dehydrogenase_(acetylating) Acetaldehyde dehydrogenase (acetylating)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.10 1.2.1.10] Full crystallographic information is available from [http:// | + | 1NVM is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Pseudomonas_sp. Pseudomonas sp.] with <scene name='pdbligand=MN:'>MN</scene>, <scene name='pdbligand=OXL:'>OXL</scene>, <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=NAD:'>NAD</scene> and <scene name='pdbligand=MPD:'>MPD</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Acetaldehyde_dehydrogenase_(acetylating) Acetaldehyde dehydrogenase (acetylating)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.10 1.2.1.10] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NVM OCA]. |
==Reference== | ==Reference== | ||
| Line 14: | Line 14: | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Pseudomonas sp.]] | [[Category: Pseudomonas sp.]] | ||
| - | [[Category: Manjasetty, A | + | [[Category: Manjasetty, A B.]] |
[[Category: Powlowski, J.]] | [[Category: Powlowski, J.]] | ||
[[Category: Vrielink, A.]] | [[Category: Vrielink, A.]] | ||
| Line 26: | Line 26: | ||
[[Category: substrate channeling]] | [[Category: substrate channeling]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:10:34 2008'' |
Revision as of 12:10, 21 February 2008
|
Crystal structure of a bifunctional aldolase-dehydrogenase : sequestering a reactive and volatile intermediate
Overview
The crystal structure of the bifunctional enzyme 4-hydroxy-2-ketovalerate aldolase (DmpG)/acylating acetaldehyde dehydrogenase (DmpF), which is involved in the bacterial degradation of toxic aromatic compounds, has been determined by multiwavelength anomalous dispersion (MAD) techniques and refined to 1.7-A resolution. Structures of the two polypeptides represent a previously unrecognized subclass of metal-dependent aldolases, and of a CoA-dependent dehydrogenase. The structure reveals a mixed state of NAD+ binding to the DmpF protomer. Domain movements associated with cofactor binding in the DmpF protomer may be correlated with channeling and activity at the DmpG protomer. In the presence of NAD+ a 29-A-long sequestered tunnel links the two active sites. Two barriers are visible along the tunnel and suggest control points for the movement of the reactive and volatile acetaldehyde intermediate between the two active sites.
About this Structure
1NVM is a Protein complex structure of sequences from Pseudomonas sp. with , , , and as ligands. Active as Acetaldehyde dehydrogenase (acetylating), with EC number 1.2.1.10 Full crystallographic information is available from OCA.
Reference
Crystal structure of a bifunctional aldolase-dehydrogenase: sequestering a reactive and volatile intermediate., Manjasetty BA, Powlowski J, Vrielink A, Proc Natl Acad Sci U S A. 2003 Jun 10;100(12):6992-7. Epub 2003 May 22. PMID:12764229
Page seeded by OCA on Thu Feb 21 14:10:34 2008
