1nvt
From Proteopedia
(New page: 200px<br /><applet load="1nvt" size="450" color="white" frame="true" align="right" spinBox="true" caption="1nvt, resolution 2.35Å" /> '''Crystal structure of...) |
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| - | [[Image:1nvt.gif|left|200px]]<br /><applet load="1nvt" size=" | + | [[Image:1nvt.gif|left|200px]]<br /><applet load="1nvt" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1nvt, resolution 2.35Å" /> | caption="1nvt, resolution 2.35Å" /> | ||
'''Crystal structure of Shikimate Dehydrogenase (AROE or MJ1084) in complex with NADP+'''<br /> | '''Crystal structure of Shikimate Dehydrogenase (AROE or MJ1084) in complex with NADP+'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystal structure of Methanococcus jannaschii shikimate | + | The crystal structure of Methanococcus jannaschii shikimate 5-dehydrogenase (MjSDH) bound to the cofactor nicotinamide adenine dinucleotide phosphate (NADP) has been determined at 2.35 A resolution. Shikimate 5-dehydrogenase (SDH) is responsible for NADP-dependent catalysis of the fourth step in shikimate biosynthesis, which is essential for aromatic amino acid metabolism in bacteria, microbial eukaryotes, and plants. The structure of MjSDH is a compact alpha/beta sandwich with two distinct domains, responsible for binding substrate and the NADP cofactor, respectively. A phylogenetically conserved deep cleft on the protein surface corresponds to the enzyme active site. The structure reveals a topologically new domain fold within the N-terminal segment of the polypeptide chain, which binds substrate and supports dimerization. Insights gained from homology modeling and sequence/structure comparisons suggest that the SDHs represent a unique class of dehydrogenases. The structure provides a framework for further investigation to discover and develop novel inhibitors targeting this essential enzyme. |
==About this Structure== | ==About this Structure== | ||
| - | 1NVT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii] with ZN and NAP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Shikimate_dehydrogenase Shikimate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.25 1.1.1.25] Full crystallographic information is available from [http:// | + | 1NVT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii] with <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=NAP:'>NAP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Shikimate_dehydrogenase Shikimate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.25 1.1.1.25] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NVT OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Shikimate dehydrogenase]] | [[Category: Shikimate dehydrogenase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Burley, S | + | [[Category: Burley, S K.]] |
| - | [[Category: NYSGXRC, New | + | [[Category: NYSGXRC, New York Structural GenomiX Research Consortium.]] |
| - | [[Category: Padyana, A | + | [[Category: Padyana, A K.]] |
[[Category: NAP]] | [[Category: NAP]] | ||
[[Category: ZN]] | [[Category: ZN]] | ||
| Line 25: | Line 25: | ||
[[Category: structural genomics]] | [[Category: structural genomics]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:10:36 2008'' |
Revision as of 12:10, 21 February 2008
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Crystal structure of Shikimate Dehydrogenase (AROE or MJ1084) in complex with NADP+
Overview
The crystal structure of Methanococcus jannaschii shikimate 5-dehydrogenase (MjSDH) bound to the cofactor nicotinamide adenine dinucleotide phosphate (NADP) has been determined at 2.35 A resolution. Shikimate 5-dehydrogenase (SDH) is responsible for NADP-dependent catalysis of the fourth step in shikimate biosynthesis, which is essential for aromatic amino acid metabolism in bacteria, microbial eukaryotes, and plants. The structure of MjSDH is a compact alpha/beta sandwich with two distinct domains, responsible for binding substrate and the NADP cofactor, respectively. A phylogenetically conserved deep cleft on the protein surface corresponds to the enzyme active site. The structure reveals a topologically new domain fold within the N-terminal segment of the polypeptide chain, which binds substrate and supports dimerization. Insights gained from homology modeling and sequence/structure comparisons suggest that the SDHs represent a unique class of dehydrogenases. The structure provides a framework for further investigation to discover and develop novel inhibitors targeting this essential enzyme.
About this Structure
1NVT is a Single protein structure of sequence from Methanocaldococcus jannaschii with and as ligands. Active as Shikimate dehydrogenase, with EC number 1.1.1.25 Full crystallographic information is available from OCA.
Reference
Crystal structure of shikimate 5-dehydrogenase (SDH) bound to NADP: insights into function and evolution., Padyana AK, Burley SK, Structure. 2003 Aug;11(8):1005-13. PMID:12906831
Page seeded by OCA on Thu Feb 21 14:10:36 2008
Categories: Methanocaldococcus jannaschii | Shikimate dehydrogenase | Single protein | Burley, S K. | NYSGXRC, New York Structural GenomiX Research Consortium. | Padyana, A K. | NAP | ZN | New york structural genomix research consortium | Nysgxrc | Protein structure initiative | Psi | Structural genomics
