1nze
From Proteopedia
(New page: 200px<br /><applet load="1nze" size="450" color="white" frame="true" align="right" spinBox="true" caption="1nze, resolution 1.95Å" /> '''Crystal structure of...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1nze.gif|left|200px]]<br /><applet load="1nze" size=" | + | [[Image:1nze.gif|left|200px]]<br /><applet load="1nze" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1nze, resolution 1.95Å" /> | caption="1nze, resolution 1.95Å" /> | ||
'''Crystal structure of PsbQ polypeptide of photosystem II from higher plants'''<br /> | '''Crystal structure of PsbQ polypeptide of photosystem II from higher plants'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The smallest extrinsic polypeptide of the water-oxidizing complex (PsbQ) | + | The smallest extrinsic polypeptide of the water-oxidizing complex (PsbQ) was extracted and purified from spinach (Spinacia oleracea) photosystem II (PSII) membranes. It was then crystallized in the presence of Zn(2+) and its structure was determined by X-ray diffraction at 1.95-A resolution using the multi-wavelength anomalous diffraction method, with the zinc as the anomalous scatterer. The crystal structure shows that the core of the protein is a four-helix bundle, whereas the amino-terminal portion, which possibly interacts with the photosystem core, is not visible in the crystal. The distribution of positive and negative charges on the protein surface might explain the ability of PsbQ to increase the binding of Cl(-) and Ca(2+) and make them available to PSII. |
==About this Structure== | ==About this Structure== | ||
| - | 1NZE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Spinacia_oleracea Spinacia oleracea] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1NZE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Spinacia_oleracea Spinacia oleracea] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NZE OCA]. |
==Reference== | ==Reference== | ||
| Line 17: | Line 17: | ||
[[Category: Battistutta, R.]] | [[Category: Battistutta, R.]] | ||
[[Category: Calderone, V.]] | [[Category: Calderone, V.]] | ||
| - | [[Category: Giacometti, G | + | [[Category: Giacometti, G M.]] |
[[Category: Trabucco, M.]] | [[Category: Trabucco, M.]] | ||
[[Category: Vujicic, A.]] | [[Category: Vujicic, A.]] | ||
| Line 28: | Line 28: | ||
[[Category: water oxidizing complex]] | [[Category: water oxidizing complex]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:11:41 2008'' |
Revision as of 12:11, 21 February 2008
|
Crystal structure of PsbQ polypeptide of photosystem II from higher plants
Overview
The smallest extrinsic polypeptide of the water-oxidizing complex (PsbQ) was extracted and purified from spinach (Spinacia oleracea) photosystem II (PSII) membranes. It was then crystallized in the presence of Zn(2+) and its structure was determined by X-ray diffraction at 1.95-A resolution using the multi-wavelength anomalous diffraction method, with the zinc as the anomalous scatterer. The crystal structure shows that the core of the protein is a four-helix bundle, whereas the amino-terminal portion, which possibly interacts with the photosystem core, is not visible in the crystal. The distribution of positive and negative charges on the protein surface might explain the ability of PsbQ to increase the binding of Cl(-) and Ca(2+) and make them available to PSII.
About this Structure
1NZE is a Single protein structure of sequence from Spinacia oleracea with as ligand. Full crystallographic information is available from OCA.
Reference
Crystal structure of the PsbQ protein of photosystem II from higher plants., Calderone V, Trabucco M, Vujicic A, Battistutta R, Giacometti GM, Andreucci F, Barbato R, Zanotti G, EMBO Rep. 2003 Sep;4(9):900-5. PMID:12949587
Page seeded by OCA on Thu Feb 21 14:11:41 2008
