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1ohu
From Proteopedia
(New page: 200px<br /><applet load="1ohu" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ohu, resolution 2.03Å" /> '''STRUCTURE OF CAENORH...) |
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| - | [[Image:1ohu.jpg|left|200px]]<br /><applet load="1ohu" size=" | + | [[Image:1ohu.jpg|left|200px]]<br /><applet load="1ohu" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1ohu, resolution 2.03Å" /> | caption="1ohu, resolution 2.03Å" /> | ||
'''STRUCTURE OF CAENORHABDITIS ELEGANS CED-9'''<br /> | '''STRUCTURE OF CAENORHABDITIS ELEGANS CED-9'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The interactions between B-cell lymphoma 2 (BCL-2) family members are | + | The interactions between B-cell lymphoma 2 (BCL-2) family members are known to be mediated through the binding of the BH3 domain of a proapoptotic member to the BH3-binding groove of an antiapoptotic member. We determined the crystal structure of antiapoptotic CED-9, which reveals a unique C-terminal helix altering the common BH3-binding region. A coexpression system to produce CED-9 in complex with proapoptotic EGL-1 enabled us to show that the binding of EGL-1 to CED-9 is extremely stable, raising the melting temperature (T(M)) of CED-9 by 25 degrees C, and that the binding surface of CED-9 extends beyond the BH3-binding region and reaches the BH4 domain. Consistently, the T(M) and a 1H-15N correlation NMR spectrum of CED-9 in complex with EGL-1 are drastically different from those of CED-9 in complex with the EGL-1 BH3 peptide. The data suggest that the recognition between other BCL-2 family members may also involve much wider protein surfaces than is previously thought. |
==About this Structure== | ==About this Structure== | ||
| - | 1OHU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http:// | + | 1OHU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OHU OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Caenorhabditis elegans]] | [[Category: Caenorhabditis elegans]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Ha, N | + | [[Category: Ha, N C.]] |
| - | [[Category: Jeong, J | + | [[Category: Jeong, J S.]] |
| - | [[Category: Oh, B | + | [[Category: Oh, B H.]] |
[[Category: apoptosis]] | [[Category: apoptosis]] | ||
[[Category: bcl-2 family]] | [[Category: bcl-2 family]] | ||
[[Category: ced-9]] | [[Category: ced-9]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:17:56 2008'' |
Revision as of 12:18, 21 February 2008
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STRUCTURE OF CAENORHABDITIS ELEGANS CED-9
Overview
The interactions between B-cell lymphoma 2 (BCL-2) family members are known to be mediated through the binding of the BH3 domain of a proapoptotic member to the BH3-binding groove of an antiapoptotic member. We determined the crystal structure of antiapoptotic CED-9, which reveals a unique C-terminal helix altering the common BH3-binding region. A coexpression system to produce CED-9 in complex with proapoptotic EGL-1 enabled us to show that the binding of EGL-1 to CED-9 is extremely stable, raising the melting temperature (T(M)) of CED-9 by 25 degrees C, and that the binding surface of CED-9 extends beyond the BH3-binding region and reaches the BH4 domain. Consistently, the T(M) and a 1H-15N correlation NMR spectrum of CED-9 in complex with EGL-1 are drastically different from those of CED-9 in complex with the EGL-1 BH3 peptide. The data suggest that the recognition between other BCL-2 family members may also involve much wider protein surfaces than is previously thought.
About this Structure
1OHU is a Single protein structure of sequence from Caenorhabditis elegans. Full crystallographic information is available from OCA.
Reference
Unique structural features of a BCL-2 family protein CED-9 and biophysical characterization of CED-9/EGL-1 interactions., Woo JS, Jung JS, Ha NC, Shin J, Kim KH, Lee W, Oh BH, Cell Death Differ. 2003 Dec;10(12):1310-9. PMID:12894216
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