1otc
From Proteopedia
(New page: 200px<br /><applet load="1otc" size="450" color="white" frame="true" align="right" spinBox="true" caption="1otc, resolution 2.80Å" /> '''THE O. NOVA TELOMERE...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1otc.gif|left|200px]]<br /><applet load="1otc" size=" | + | [[Image:1otc.gif|left|200px]]<br /><applet load="1otc" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1otc, resolution 2.80Å" /> | caption="1otc, resolution 2.80Å" /> | ||
'''THE O. NOVA TELOMERE END BINDING PROTEIN COMPLEXED WITH SINGLE STRAND DNA'''<br /> | '''THE O. NOVA TELOMERE END BINDING PROTEIN COMPLEXED WITH SINGLE STRAND DNA'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Telomeres are specialized protein-DNA complexes that compose the ends of | + | Telomeres are specialized protein-DNA complexes that compose the ends of eukaryotic chromosomes. Telomeres protect chromosome termini from degradation and recombination and act together with telomerase to ensure complete genome replication. We have determined the crystal structure of the two-subunit Oxytricha nova telomere end binding protein (OnTEBP) complexed with single strand telomeric DNA at 2.8 A resolution. The structure reveals four oligonucleotide/oligosaccharide-binding folds, three of which form a deep cleft that binds the ssDNA, and a fourth that forms an unusual protein-protein interaction between the alpha and beta subunits. This structure provides a molecular description of how the two subunits of OnTEBP recognize and bind ssDNA to form a sequence-specific, telomeric nucleoprotein complex that caps the very 3' ends of chromosomes. |
==About this Structure== | ==About this Structure== | ||
| - | 1OTC is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Sterkiella_nova Sterkiella nova]. Full crystallographic information is available from [http:// | + | 1OTC is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Sterkiella_nova Sterkiella nova]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OTC OCA]. |
==Reference== | ==Reference== | ||
| Line 13: | Line 13: | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Sterkiella nova]] | [[Category: Sterkiella nova]] | ||
| - | [[Category: Bevilacqua, J | + | [[Category: Bevilacqua, J M.]] |
| - | [[Category: Horvath, M | + | [[Category: Horvath, M P.]] |
| - | [[Category: Ruggles, J | + | [[Category: Ruggles, J A.]] |
| - | [[Category: Schultz, S | + | [[Category: Schultz, S C.]] |
| - | [[Category: Schweiker, V | + | [[Category: Schweiker, V L.]] |
[[Category: ob fold]] | [[Category: ob fold]] | ||
[[Category: oligonucleotide and oligosaccharide binding fold]] | [[Category: oligonucleotide and oligosaccharide binding fold]] | ||
| Line 25: | Line 25: | ||
[[Category: telomeres]] | [[Category: telomeres]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:21:26 2008'' |
Revision as of 12:21, 21 February 2008
|
THE O. NOVA TELOMERE END BINDING PROTEIN COMPLEXED WITH SINGLE STRAND DNA
Overview
Telomeres are specialized protein-DNA complexes that compose the ends of eukaryotic chromosomes. Telomeres protect chromosome termini from degradation and recombination and act together with telomerase to ensure complete genome replication. We have determined the crystal structure of the two-subunit Oxytricha nova telomere end binding protein (OnTEBP) complexed with single strand telomeric DNA at 2.8 A resolution. The structure reveals four oligonucleotide/oligosaccharide-binding folds, three of which form a deep cleft that binds the ssDNA, and a fourth that forms an unusual protein-protein interaction between the alpha and beta subunits. This structure provides a molecular description of how the two subunits of OnTEBP recognize and bind ssDNA to form a sequence-specific, telomeric nucleoprotein complex that caps the very 3' ends of chromosomes.
About this Structure
1OTC is a Protein complex structure of sequences from Sterkiella nova. Full crystallographic information is available from OCA.
Reference
Crystal structure of the Oxytricha nova telomere end binding protein complexed with single strand DNA., Horvath MP, Schweiker VL, Bevilacqua JM, Ruggles JA, Schultz SC, Cell. 1998 Dec 23;95(7):963-74. PMID:9875850
Page seeded by OCA on Thu Feb 21 14:21:26 2008
