1ova
From Proteopedia
(New page: 200px<br /><applet load="1ova" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ova, resolution 1.95Å" /> '''CRYSTAL STRUCTURE OF...) |
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| - | [[Image:1ova.gif|left|200px]]<br /><applet load="1ova" size=" | + | [[Image:1ova.gif|left|200px]]<br /><applet load="1ova" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1ova, resolution 1.95Å" /> | caption="1ova, resolution 1.95Å" /> | ||
'''CRYSTAL STRUCTURE OF UNCLEAVED OVALBUMIN AT 1.95 ANGSTROMS RESOLUTION'''<br /> | '''CRYSTAL STRUCTURE OF UNCLEAVED OVALBUMIN AT 1.95 ANGSTROMS RESOLUTION'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Ovalbumin, the major protein in avian egg-white, is a non-inhibitory | + | Ovalbumin, the major protein in avian egg-white, is a non-inhibitory member of the serine protease inhibitor (serpin) superfamily. The crystal structure of uncleaved, hen ovalbumin was solved by the molecular replacement method using the structure of plakalbumin, a proteolytically cleaved form of ovalbumin, as a starting model. The final refined model, including four ovalbumin molecules, 678 water molecules and a single metal ion, has a crystallographic R-factor of 17.4% for all reflections between 6.0 and 1.95 A resolution. The root-mean-square deviation from ideal values in bond lengths is 0.02 A and in bond angles is 2.9 degrees. This is the first crystal structure of a member of the serpin family in an uncleaved form. Surprisingly, the peptide that is homologous to the reactive centre of inhibitory serpins adopts an alpha-helical conformation. The implications for the mechanism of inhibition of the inhibitory members of the family is discussed. |
==About this Structure== | ==About this Structure== | ||
| - | 1OVA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] with NAG, PO3, CA and ACE as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1OVA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] with <scene name='pdbligand=NAG:'>NAG</scene>, <scene name='pdbligand=PO3:'>PO3</scene>, <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=ACE:'>ACE</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OVA OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Gallus gallus]] | [[Category: Gallus gallus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Leslie, A | + | [[Category: Leslie, A G.W.]] |
| - | [[Category: Stein, P | + | [[Category: Stein, P E.]] |
[[Category: ACE]] | [[Category: ACE]] | ||
[[Category: CA]] | [[Category: CA]] | ||
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[[Category: serpin]] | [[Category: serpin]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:21:56 2008'' |
Revision as of 12:21, 21 February 2008
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CRYSTAL STRUCTURE OF UNCLEAVED OVALBUMIN AT 1.95 ANGSTROMS RESOLUTION
Overview
Ovalbumin, the major protein in avian egg-white, is a non-inhibitory member of the serine protease inhibitor (serpin) superfamily. The crystal structure of uncleaved, hen ovalbumin was solved by the molecular replacement method using the structure of plakalbumin, a proteolytically cleaved form of ovalbumin, as a starting model. The final refined model, including four ovalbumin molecules, 678 water molecules and a single metal ion, has a crystallographic R-factor of 17.4% for all reflections between 6.0 and 1.95 A resolution. The root-mean-square deviation from ideal values in bond lengths is 0.02 A and in bond angles is 2.9 degrees. This is the first crystal structure of a member of the serpin family in an uncleaved form. Surprisingly, the peptide that is homologous to the reactive centre of inhibitory serpins adopts an alpha-helical conformation. The implications for the mechanism of inhibition of the inhibitory members of the family is discussed.
About this Structure
1OVA is a Single protein structure of sequence from Gallus gallus with , , and as ligands. Full crystallographic information is available from OCA.
Reference
Crystal structure of uncleaved ovalbumin at 1.95 A resolution., Stein PE, Leslie AG, Finch JT, Carrell RW, J Mol Biol. 1991 Oct 5;221(3):941-59. PMID:1942038
Page seeded by OCA on Thu Feb 21 14:21:56 2008
Categories: Gallus gallus | Single protein | Leslie, A G.W. | Stein, P E. | ACE | CA | NAG | PO3 | Serpin
