1oyu
From Proteopedia
(New page: 200px<br /><applet load="1oyu" size="450" color="white" frame="true" align="right" spinBox="true" caption="1oyu, resolution 2.5Å" /> '''Long-Distance conform...) |
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| - | [[Image:1oyu.jpg|left|200px]]<br /><applet load="1oyu" size=" | + | [[Image:1oyu.jpg|left|200px]]<br /><applet load="1oyu" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1oyu, resolution 2.5Å" /> | caption="1oyu, resolution 2.5Å" /> | ||
'''Long-Distance conformational changes in a protein engineered by modulated sequence duplication'''<br /> | '''Long-Distance conformational changes in a protein engineered by modulated sequence duplication'''<br /> | ||
==Overview== | ==Overview== | ||
| - | There are few, if any, known instances in which a biological signal is | + | There are few, if any, known instances in which a biological signal is transmitted via a large conformational change through the body of a protein. We describe here a mutant of T4 lysozyme that was engineered to permit structural change at a distance. The design uses a tandem sequence repeat that makes it possible to transmit large-scale structural changes from one end of an alpha-helix to the other over a distance of 17-25 A. The method should be of general applicability and may make it possible to introduce a mutation at one site in a protein that will induce large-scale changes in the structure at a spatially remote site. |
==About this Structure== | ==About this Structure== | ||
| - | 1OYU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t4 Bacteriophage t4]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Full crystallographic information is available from [http:// | + | 1OYU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t4 Bacteriophage t4]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OYU OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Gay, L.]] | [[Category: Gay, L.]] | ||
| - | [[Category: Matthews, B | + | [[Category: Matthews, B W.]] |
[[Category: Sagermann, M.]] | [[Category: Sagermann, M.]] | ||
[[Category: design of structural switches]] | [[Category: design of structural switches]] | ||
| Line 22: | Line 22: | ||
[[Category: tandem repeat]] | [[Category: tandem repeat]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:23:17 2008'' |
Revision as of 12:23, 21 February 2008
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Long-Distance conformational changes in a protein engineered by modulated sequence duplication
Overview
There are few, if any, known instances in which a biological signal is transmitted via a large conformational change through the body of a protein. We describe here a mutant of T4 lysozyme that was engineered to permit structural change at a distance. The design uses a tandem sequence repeat that makes it possible to transmit large-scale structural changes from one end of an alpha-helix to the other over a distance of 17-25 A. The method should be of general applicability and may make it possible to introduce a mutation at one site in a protein that will induce large-scale changes in the structure at a spatially remote site.
About this Structure
1OYU is a Single protein structure of sequence from Bacteriophage t4. Active as Lysozyme, with EC number 3.2.1.17 Full crystallographic information is available from OCA.
Reference
Long-distance conformational changes in a protein engineered by modulated sequence duplication., Sagermann M, Gay L, Matthews BW, Proc Natl Acad Sci U S A. 2003 Aug 5;100(16):9191-5. Epub 2003 Jul 17. PMID:12869697
Page seeded by OCA on Thu Feb 21 14:23:17 2008
