1p5d
From Proteopedia
(New page: 200px<br /><applet load="1p5d" size="450" color="white" frame="true" align="right" spinBox="true" caption="1p5d, resolution 1.60Å" /> '''Enzyme-ligand comple...) |
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| - | [[Image:1p5d.jpg|left|200px]]<br /><applet load="1p5d" size=" | + | [[Image:1p5d.jpg|left|200px]]<br /><applet load="1p5d" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1p5d, resolution 1.60Å" /> | caption="1p5d, resolution 1.60Å" /> | ||
'''Enzyme-ligand complex of P. aeruginosa PMM/PGM'''<br /> | '''Enzyme-ligand complex of P. aeruginosa PMM/PGM'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Enzyme-substrate complexes of phosphomannomutase/phosphoglucomutase | + | Enzyme-substrate complexes of phosphomannomutase/phosphoglucomutase (PMM/PGM) reveal the structural basis of the enzyme's ability to use four different substrates in catalysis. High-resolution structures with glucose 1-phosphate, glucose 6-phosphate, mannose 1-phosphate, and mannose 6-phosphate show that the position of the phosphate group of each substrate is held constant by a conserved network of hydrogen bonds. This produces two distinct, and mutually exclusive, binding orientations for the sugar rings of the 1-phospho and 6-phospho sugars. Specific binding of both orientations is accomplished by key contacts with the O3 and O4 hydroxyls of the sugar, which must occupy equatorial positions. Dual recognition of glucose and mannose phosphosugars uses a combination of specific protein contacts and nonspecific solvent contacts. The ability of PMM/PGM to accommodate these four diverse substrates in a single active site is consistent with its highly reversible phosphoryl transfer reaction and allows it to function in multiple biosynthetic pathways in P. aeruginosa. |
==About this Structure== | ==About this Structure== | ||
| - | 1P5D is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] with G1P and ZN as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Phosphomannomutase Phosphomannomutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.2.8 5.4.2.8] Full crystallographic information is available from [http:// | + | 1P5D is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] with <scene name='pdbligand=G1P:'>G1P</scene> and <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Phosphomannomutase Phosphomannomutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.2.8 5.4.2.8] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P5D OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Pseudomonas aeruginosa]] | [[Category: Pseudomonas aeruginosa]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Beamer, L | + | [[Category: Beamer, L J.]] |
[[Category: Regni, C.]] | [[Category: Regni, C.]] | ||
| - | [[Category: Tipton, P | + | [[Category: Tipton, P A.]] |
[[Category: G1P]] | [[Category: G1P]] | ||
[[Category: ZN]] | [[Category: ZN]] | ||
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[[Category: phosphoserine]] | [[Category: phosphoserine]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:25:16 2008'' |
Revision as of 12:25, 21 February 2008
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Enzyme-ligand complex of P. aeruginosa PMM/PGM
Overview
Enzyme-substrate complexes of phosphomannomutase/phosphoglucomutase (PMM/PGM) reveal the structural basis of the enzyme's ability to use four different substrates in catalysis. High-resolution structures with glucose 1-phosphate, glucose 6-phosphate, mannose 1-phosphate, and mannose 6-phosphate show that the position of the phosphate group of each substrate is held constant by a conserved network of hydrogen bonds. This produces two distinct, and mutually exclusive, binding orientations for the sugar rings of the 1-phospho and 6-phospho sugars. Specific binding of both orientations is accomplished by key contacts with the O3 and O4 hydroxyls of the sugar, which must occupy equatorial positions. Dual recognition of glucose and mannose phosphosugars uses a combination of specific protein contacts and nonspecific solvent contacts. The ability of PMM/PGM to accommodate these four diverse substrates in a single active site is consistent with its highly reversible phosphoryl transfer reaction and allows it to function in multiple biosynthetic pathways in P. aeruginosa.
About this Structure
1P5D is a Single protein structure of sequence from Pseudomonas aeruginosa with and as ligands. Active as Phosphomannomutase, with EC number 5.4.2.8 Full crystallographic information is available from OCA.
Reference
Structural basis of diverse substrate recognition by the enzyme PMM/PGM from P. aeruginosa., Regni C, Naught L, Tipton PA, Beamer LJ, Structure. 2004 Jan;12(1):55-63. PMID:14725765
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