1p5u
From Proteopedia
(New page: 200px<br /><applet load="1p5u" size="450" color="white" frame="true" align="right" spinBox="true" caption="1p5u, resolution 1.99Å" /> '''X-ray structure of t...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1p5u.jpg|left|200px]]<br /><applet load="1p5u" size=" | + | [[Image:1p5u.jpg|left|200px]]<br /><applet load="1p5u" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1p5u, resolution 1.99Å" /> | caption="1p5u, resolution 1.99Å" /> | ||
'''X-ray structure of the ternary Caf1M:Caf1:Caf1 chaperone:subunit:subunit complex'''<br /> | '''X-ray structure of the ternary Caf1M:Caf1:Caf1 chaperone:subunit:subunit complex'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Most gram-negative pathogens express fibrous adhesive virulence organelles | + | Most gram-negative pathogens express fibrous adhesive virulence organelles that mediate targeting to the sites of infection. The F1 capsular antigen from the plague pathogen Yersinia pestis consists of linear fibers of a single subunit (Caf1) and serves as a prototype for nonpilus organelles assembled via the chaperone/usher pathway. Genetic data together with high-resolution X-ray structures corresponding to snapshots of the assembly process reveal the structural basis of fiber formation. Comparison of chaperone bound Caf1 subunit with the subunit in the fiber reveals a novel type of conformational change involving the entire hydrophobic core of the protein. The observed conformational change suggests that the chaperone traps a high-energy folding intermediate of Caf1. A model is proposed in which release of the subunit allows folding to be completed, driving fiber formation. |
==About this Structure== | ==About this Structure== | ||
| - | 1P5U is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Yersinia_pestis Yersinia pestis]. Full crystallographic information is available from [http:// | + | 1P5U is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Yersinia_pestis Yersinia pestis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P5U OCA]. |
==Reference== | ==Reference== | ||
| Line 14: | Line 14: | ||
[[Category: Yersinia pestis]] | [[Category: Yersinia pestis]] | ||
[[Category: Berglund, J.]] | [[Category: Berglund, J.]] | ||
| - | [[Category: Fooks, L | + | [[Category: Fooks, L J.]] |
| - | [[Category: Ibrahim, T | + | [[Category: Ibrahim, T M.]] |
| - | [[Category: Knight, S | + | [[Category: Knight, S D.]] |
[[Category: MacIntyre, S.]] | [[Category: MacIntyre, S.]] | ||
| - | [[Category: Pudney, A | + | [[Category: Pudney, A F.]] |
| - | [[Category: Zavialov, A | + | [[Category: Zavialov, A V.]] |
[[Category: chaperone]] | [[Category: chaperone]] | ||
[[Category: chaperone-subunit complex]] | [[Category: chaperone-subunit complex]] | ||
| Line 27: | Line 27: | ||
[[Category: protein fiber]] | [[Category: protein fiber]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:25:24 2008'' |
Revision as of 12:25, 21 February 2008
|
X-ray structure of the ternary Caf1M:Caf1:Caf1 chaperone:subunit:subunit complex
Overview
Most gram-negative pathogens express fibrous adhesive virulence organelles that mediate targeting to the sites of infection. The F1 capsular antigen from the plague pathogen Yersinia pestis consists of linear fibers of a single subunit (Caf1) and serves as a prototype for nonpilus organelles assembled via the chaperone/usher pathway. Genetic data together with high-resolution X-ray structures corresponding to snapshots of the assembly process reveal the structural basis of fiber formation. Comparison of chaperone bound Caf1 subunit with the subunit in the fiber reveals a novel type of conformational change involving the entire hydrophobic core of the protein. The observed conformational change suggests that the chaperone traps a high-energy folding intermediate of Caf1. A model is proposed in which release of the subunit allows folding to be completed, driving fiber formation.
About this Structure
1P5U is a Protein complex structure of sequences from Yersinia pestis. Full crystallographic information is available from OCA.
Reference
Structure and biogenesis of the capsular F1 antigen from Yersinia pestis: preserved folding energy drives fiber formation., Zavialov AV, Berglund J, Pudney AF, Fooks LJ, Ibrahim TM, MacIntyre S, Knight SD, Cell. 2003 May 30;113(5):587-96. PMID:12787500
Page seeded by OCA on Thu Feb 21 14:25:24 2008
