1p6r
From Proteopedia
(New page: 200px<br /><applet load="1p6r" size="450" color="white" frame="true" align="right" spinBox="true" caption="1p6r" /> '''Solution structure of the DNA binding domain...) |
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| - | [[Image:1p6r.jpg|left|200px]]<br /><applet load="1p6r" size=" | + | [[Image:1p6r.jpg|left|200px]]<br /><applet load="1p6r" size="350" color="white" frame="true" align="right" spinBox="true" |
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'''Solution structure of the DNA binding domain of the repressor BlaI.'''<br /> | '''Solution structure of the DNA binding domain of the repressor BlaI.'''<br /> | ||
==Overview== | ==Overview== | ||
| - | beta-Lactamase and penicillin-binding protein PBP2' mediate staphylococcal | + | beta-Lactamase and penicillin-binding protein PBP2' mediate staphylococcal resistance to beta-lactam antibiotics, which are otherwise highly clinically effective. Two repressors (BlaI and MecI) regulate expression of these inducible proteins. Here, we present the first solution structure of the 82 amino acid residue DNA-binding domain of Bacillus licheniformis BlaI which is very similar in primary sequence to the medically significant Staphyloccocal BlaI and MecI proteins. This structure is composed of a compact core of three alpha-helices and a three-stranded beta-sheet typical of the winged helix protein (WHP) family. The protein/DNA complex was studied by NMR chemical shift comparison between the free and complexed forms of BlaI. Residues involved in DNA interaction were identified and a WHP canonical model of interaction with the operators is proposed. In this model, specific contacts occur between the base-pairs of the TACA motif and conserved amino acid residues of the repressor helix H3. These results help toward understanding the repression and induction mechanism of the genes coding for beta-lactamase and PBP2'. |
==About this Structure== | ==About this Structure== | ||
| - | 1P6R is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_licheniformis Bacillus licheniformis]. Full crystallographic information is available from [http:// | + | 1P6R is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_licheniformis Bacillus licheniformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P6R OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Joris, B.]] | [[Category: Joris, B.]] | ||
[[Category: Llabres, G.]] | [[Category: Llabres, G.]] | ||
| - | [[Category: Melckebeke, H | + | [[Category: Melckebeke, H V.]] |
| - | [[Category: Simorre, J | + | [[Category: Simorre, J P.]] |
[[Category: Vreuls, C.]] | [[Category: Vreuls, C.]] | ||
[[Category: bacterial resistance to antibiotics]] | [[Category: bacterial resistance to antibiotics]] | ||
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[[Category: winged helix protein]] | [[Category: winged helix protein]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:25:44 2008'' |
Revision as of 12:25, 21 February 2008
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Solution structure of the DNA binding domain of the repressor BlaI.
Overview
beta-Lactamase and penicillin-binding protein PBP2' mediate staphylococcal resistance to beta-lactam antibiotics, which are otherwise highly clinically effective. Two repressors (BlaI and MecI) regulate expression of these inducible proteins. Here, we present the first solution structure of the 82 amino acid residue DNA-binding domain of Bacillus licheniformis BlaI which is very similar in primary sequence to the medically significant Staphyloccocal BlaI and MecI proteins. This structure is composed of a compact core of three alpha-helices and a three-stranded beta-sheet typical of the winged helix protein (WHP) family. The protein/DNA complex was studied by NMR chemical shift comparison between the free and complexed forms of BlaI. Residues involved in DNA interaction were identified and a WHP canonical model of interaction with the operators is proposed. In this model, specific contacts occur between the base-pairs of the TACA motif and conserved amino acid residues of the repressor helix H3. These results help toward understanding the repression and induction mechanism of the genes coding for beta-lactamase and PBP2'.
About this Structure
1P6R is a Single protein structure of sequence from Bacillus licheniformis. Full crystallographic information is available from OCA.
Reference
Solution structural study of BlaI: implications for the repression of genes involved in beta-lactam antibiotic resistance., Melckebeke HV, Vreuls C, Gans P, Filee P, Llabres G, Joris B, Simorre JP, J Mol Biol. 2003 Oct 31;333(4):711-20. PMID:14568532
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