1prv
From Proteopedia
(New page: 200px<br /><applet load="1prv" size="450" color="white" frame="true" align="right" spinBox="true" caption="1prv" /> '''PURINE REPRESSOR DNA-BINDING DOMAIN DNA BIND...) |
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| - | [[Image:1prv.jpg|left|200px]]<br /><applet load="1prv" size=" | + | [[Image:1prv.jpg|left|200px]]<br /><applet load="1prv" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1prv" /> | caption="1prv" /> | ||
'''PURINE REPRESSOR DNA-BINDING DOMAIN DNA BINDING'''<br /> | '''PURINE REPRESSOR DNA-BINDING DOMAIN DNA BINDING'''<br /> | ||
==Overview== | ==Overview== | ||
| - | BACKGROUND: The purine repressor (PurR) regulates genes that encode | + | BACKGROUND: The purine repressor (PurR) regulates genes that encode enzymes for purine biosynthesis. PurR has a two domain structure with an N-terminal DNA-binding domain (DBD) and a C-terminal corepressor-binding domain (CBD). The three dimensional structure of a ternary complex of PurR bound to both corepressor and a specific DNA sequence has recently been determined by X-ray crystallography. RESULTS: We have determined the solution structure of the PurR DBD by NMR. It contains three helices, with the first and second helices forming a helix-turn-helix motif. The tertiary structure of the three helices is very similar to that of the corresponding region in the ternary complex. The structure of the hinge helical region, however, which makes specific base contacts in the minor groove of DNA, is disordered in the DNA-free form. CONCLUSION: The stable formation of PurR hinge helices requires PurR dimerization, which brings the hinge regions proximal to each other. The dimerization of the hinge helices is likely to be controlled by the CBD dimerization interface, but is induced by specific-DNA binding. |
==About this Structure== | ==About this Structure== | ||
| - | 1PRV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http:// | + | 1PRV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PRV OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Brennan, R | + | [[Category: Brennan, R G.]] |
[[Category: Enari, M.]] | [[Category: Enari, M.]] | ||
[[Category: Kobayashi, K.]] | [[Category: Kobayashi, K.]] | ||
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[[Category: Nishimura, Y.]] | [[Category: Nishimura, Y.]] | ||
[[Category: Sampei, G.]] | [[Category: Sampei, G.]] | ||
| - | [[Category: Schumacher, M | + | [[Category: Schumacher, M A.]] |
[[Category: Yamamoto, H.]] | [[Category: Yamamoto, H.]] | ||
[[Category: purine repressor]] | [[Category: purine repressor]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:31:58 2008'' |
Revision as of 12:31, 21 February 2008
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PURINE REPRESSOR DNA-BINDING DOMAIN DNA BINDING
Overview
BACKGROUND: The purine repressor (PurR) regulates genes that encode enzymes for purine biosynthesis. PurR has a two domain structure with an N-terminal DNA-binding domain (DBD) and a C-terminal corepressor-binding domain (CBD). The three dimensional structure of a ternary complex of PurR bound to both corepressor and a specific DNA sequence has recently been determined by X-ray crystallography. RESULTS: We have determined the solution structure of the PurR DBD by NMR. It contains three helices, with the first and second helices forming a helix-turn-helix motif. The tertiary structure of the three helices is very similar to that of the corresponding region in the ternary complex. The structure of the hinge helical region, however, which makes specific base contacts in the minor groove of DNA, is disordered in the DNA-free form. CONCLUSION: The stable formation of PurR hinge helices requires PurR dimerization, which brings the hinge regions proximal to each other. The dimerization of the hinge helices is likely to be controlled by the CBD dimerization interface, but is induced by specific-DNA binding.
About this Structure
1PRV is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Structural comparison of the free and DNA-bound forms of the purine repressor DNA-binding domain., Nagadoi A, Morikawa S, Nakamura H, Enari M, Kobayashi K, Yamamoto H, Sampei G, Mizobuchi K, Schumacher MA, Brennan RG, et al., Structure. 1995 Nov 15;3(11):1217-24. PMID:8591032
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