1psf
From Proteopedia
(New page: 200px<br /><applet load="1psf" size="450" color="white" frame="true" align="right" spinBox="true" caption="1psf" /> '''THE THREE-DIMENSIONAL SOLUTION STRUCTURE OF ...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1psf.gif|left|200px]]<br /><applet load="1psf" size=" | + | [[Image:1psf.gif|left|200px]]<br /><applet load="1psf" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1psf" /> | caption="1psf" /> | ||
'''THE THREE-DIMENSIONAL SOLUTION STRUCTURE OF PSAE FROM THE CYANOBACTERIUM SYNECHOCOCCUS SP. STRAIN PCC 7002: A PHOTOSYSTEM I PROTEIN THAT SHOWS STRUCTURAL HOMOLOGY WITH SH3 DOMAINS'''<br /> | '''THE THREE-DIMENSIONAL SOLUTION STRUCTURE OF PSAE FROM THE CYANOBACTERIUM SYNECHOCOCCUS SP. STRAIN PCC 7002: A PHOTOSYSTEM I PROTEIN THAT SHOWS STRUCTURAL HOMOLOGY WITH SH3 DOMAINS'''<br /> | ||
==Overview== | ==Overview== | ||
| - | PsaE is a 69 amino acid polypeptide from photosystem I present on the | + | PsaE is a 69 amino acid polypeptide from photosystem I present on the stromal side of the thylakoid membrane. The three-dimensional solution structure of this protein from the cyanobacterium Synechococcus sp. strain PCC 7002 was determined at pH 5.8 and room temperature using over 900 experimental restraints derived from two- and three-dimensional NMR experiments. The structure is comprised of a well-defined five-stranded beta-sheet with (+1, +1, +1, -4 alpha) topology. There is no helical region except for a single turn of 3(10) helix between the beta D and beta E strands. PsaE also exhibits a large unrestrained loop spanning residues 42-56. A comparison to known protein structures revealed similarity with the Src homology 3 (SH3) domain, a membrane-associated protein involved in signal transduction in eukaryotes. The match is remarkable as 47 of the alpha-carbons of PsaE can be superimposed onto those of the SH3 domain from chicken brain alpha-spectrin with a root-mean-square deviation of 2.3 A. Although the amino acid sequences have low identity and the loops are different in both proteins, the topology of the beta-sheet and the 3(10) turn is conserved. SH3 domains from other sources show a similar structural homology. The structure of PsaE was used to suggest approaches for elucidating its roles within photosystem I. |
==About this Structure== | ==About this Structure== | ||
| - | 1PSF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Synechococcus_sp. Synechococcus sp.]. Full crystallographic information is available from [http:// | + | 1PSF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Synechococcus_sp. Synechococcus sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PSF OCA]. |
==Reference== | ==Reference== | ||
| Line 13: | Line 13: | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Synechococcus sp.]] | [[Category: Synechococcus sp.]] | ||
| - | [[Category: Bryant, D | + | [[Category: Bryant, D A.]] |
| - | [[Category: Falzone, C | + | [[Category: Falzone, C J.]] |
| - | [[Category: Kao, Y | + | [[Category: Kao, Y H.]] |
| - | [[Category: Lecomte, J | + | [[Category: Lecomte, J T.J.]] |
[[Category: Zhao, J.]] | [[Category: Zhao, J.]] | ||
[[Category: photosystem i]] | [[Category: photosystem i]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:32:03 2008'' |
Revision as of 12:32, 21 February 2008
|
THE THREE-DIMENSIONAL SOLUTION STRUCTURE OF PSAE FROM THE CYANOBACTERIUM SYNECHOCOCCUS SP. STRAIN PCC 7002: A PHOTOSYSTEM I PROTEIN THAT SHOWS STRUCTURAL HOMOLOGY WITH SH3 DOMAINS
Overview
PsaE is a 69 amino acid polypeptide from photosystem I present on the stromal side of the thylakoid membrane. The three-dimensional solution structure of this protein from the cyanobacterium Synechococcus sp. strain PCC 7002 was determined at pH 5.8 and room temperature using over 900 experimental restraints derived from two- and three-dimensional NMR experiments. The structure is comprised of a well-defined five-stranded beta-sheet with (+1, +1, +1, -4 alpha) topology. There is no helical region except for a single turn of 3(10) helix between the beta D and beta E strands. PsaE also exhibits a large unrestrained loop spanning residues 42-56. A comparison to known protein structures revealed similarity with the Src homology 3 (SH3) domain, a membrane-associated protein involved in signal transduction in eukaryotes. The match is remarkable as 47 of the alpha-carbons of PsaE can be superimposed onto those of the SH3 domain from chicken brain alpha-spectrin with a root-mean-square deviation of 2.3 A. Although the amino acid sequences have low identity and the loops are different in both proteins, the topology of the beta-sheet and the 3(10) turn is conserved. SH3 domains from other sources show a similar structural homology. The structure of PsaE was used to suggest approaches for elucidating its roles within photosystem I.
About this Structure
1PSF is a Single protein structure of sequence from Synechococcus sp.. Full crystallographic information is available from OCA.
Reference
Three-dimensional solution structure of PsaE from the cyanobacterium Synechococcus sp. strain PCC 7002, a photosystem I protein that shows structural homology with SH3 domains., Falzone CJ, Kao YH, Zhao J, Bryant DA, Lecomte JT, Biochemistry. 1994 May 24;33(20):6052-62. PMID:8193119
Page seeded by OCA on Thu Feb 21 14:32:03 2008
