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1odm
From Proteopedia
(New page: 200px<br /> <applet load="1odm" size="450" color="white" frame="true" align="right" spinBox="true" caption="1odm, resolution 1.15Å" /> '''ISOPENICILLIN N SYN...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1ODM is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Emericella_nidulans Emericella nidulans]] with SO4, FE2 and ASV as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.21.3.1 1.21.3.1]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1ODM OCA]]. | + | 1ODM is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Emericella_nidulans Emericella nidulans]] with SO4, FE2 and ASV as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Isopenicillin-N_synthase Isopenicillin-N synthase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.21.3.1 1.21.3.1]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1ODM OCA]]. |
==Reference== | ==Reference== | ||
Crystallographic studies on the reaction of isopenicillin N synthase with an unsaturated substrate analogue., Elkins JM, Rutledge PJ, Burzlaff NI, Clifton IJ, Adlington RM, Roach PL, Baldwin JE, Org Biomol Chem. 2003 May 7;1(9):1455-60. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12926272 12926272] | Crystallographic studies on the reaction of isopenicillin N synthase with an unsaturated substrate analogue., Elkins JM, Rutledge PJ, Burzlaff NI, Clifton IJ, Adlington RM, Roach PL, Baldwin JE, Org Biomol Chem. 2003 May 7;1(9):1455-60. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12926272 12926272] | ||
[[Category: Emericella nidulans]] | [[Category: Emericella nidulans]] | ||
| + | [[Category: Isopenicillin-N synthase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Adlington, R.M.]] | [[Category: Adlington, R.M.]] | ||
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[[Category: penicillin biosynthesis]] | [[Category: penicillin biosynthesis]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 13:35:09 2007'' |
Revision as of 11:30, 30 October 2007
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ISOPENICILLIN N SYNTHASE FROM ASPERGILLUS NIDULANS (ANAEROBIC AC-VINYLGLYCINE FE COMPLEX)
Overview
Isopenicillin N synthase (IPNS) catalyses conversion of the linear, tripeptide delta-(L-alpha-aminoadipoyl)-L-cysteinyl-D-valine (ACV) to, isopenicillin N (IPN), the central step in biosynthesis of the beta-lactam, antibiotics. The unsaturated substrate analogue, delta-(L-alpha-aminoadipoyl)-L-cysteinyl-D-vinylglycine (ACvG) has, previously been incubated with IPNS and single product was isolated, a, 2-alpha-hydroxymethyl isopenicillin N (HMPen), formed via a monooxygenase, mode of reactivity. ACvG has now been crystallised with IPNS and the, structure of the anaerobic IPNS:Fe(II):ACvG complex determined to 1.15 A, resolution. Furthermore, by exposing the anaerobically grown crystals to, high-pressure oxygen gas, a structure corresponding to the bicyclic, product HMPen has been obtained ... [(full description)]
About this Structure
1ODM is a [Single protein] structure of sequence from [Emericella nidulans] with SO4, FE2 and ASV as [ligands]. Active as [Isopenicillin-N synthase], with EC number [1.21.3.1]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
Crystallographic studies on the reaction of isopenicillin N synthase with an unsaturated substrate analogue., Elkins JM, Rutledge PJ, Burzlaff NI, Clifton IJ, Adlington RM, Roach PL, Baldwin JE, Org Biomol Chem. 2003 May 7;1(9):1455-60. PMID:12926272
Page seeded by OCA on Tue Oct 30 13:35:09 2007
Categories: Emericella nidulans | Isopenicillin-N synthase | Single protein | Adlington, R.M. | Baldwin, J.E. | Burzlaff, N.I. | Clifton, I.J. | Elkins, J.M. | Roach, P.L. | Rutledge, P.J. | ASV | FE2 | SO4 | Antibiotic biosynthesis | B-lactam antibiotic | Iron | Oxidoreductase | Oxygenase | Penicillin biosynthesis
