1pxe
From Proteopedia
(New page: 200px<br /><applet load="1pxe" size="450" color="white" frame="true" align="right" spinBox="true" caption="1pxe" /> '''Solution Structure of a CCHHC Domain of Neur...) |
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| - | [[Image:1pxe.gif|left|200px]]<br /><applet load="1pxe" size=" | + | [[Image:1pxe.gif|left|200px]]<br /><applet load="1pxe" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1pxe" /> | caption="1pxe" /> | ||
'''Solution Structure of a CCHHC Domain of Neural Zinc Finger Factor-1'''<br /> | '''Solution Structure of a CCHHC Domain of Neural Zinc Finger Factor-1'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The structure of a CCHHC zinc-binding domain from neural zinc finger | + | The structure of a CCHHC zinc-binding domain from neural zinc finger factor-1 (NZF-1) has been determined in solution though the use of NMR methods. This domain is a member of a family of domains that have the Cys-X(4)-Cys-X(4)-His-X(7)-His-X(5)-Cys consensus sequence. The structure determination reveals a novel fold based around a zinc(II) ion coordinated to three Cys residues and the second of the two conserved His residues. The other His residue is stacked between the metal-coordinated His residue and a relatively conserved aromatic residue. Analysis of His to Gln sequence variants reveals that both His residues are required for the formation of a well-defined structure, but neither is required for high-affinity metal binding at a tetrahedral site. The structure suggests that a two-domain protein fragment and a double-stranded DNA binding site may interact with a common two-fold axis relating the two domains and the two half-sites of the DNA-inverted repeat. |
==About this Structure== | ==About this Structure== | ||
| - | 1PXE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1PXE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PXE OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Rattus norvegicus]] | [[Category: Rattus norvegicus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Amann, B | + | [[Category: Amann, B T.]] |
| - | [[Category: Berg, J | + | [[Category: Berg, J M.]] |
| - | [[Category: Berkovits-Cymet, H | + | [[Category: Berkovits-Cymet, H J.]] |
[[Category: ZN]] | [[Category: ZN]] | ||
[[Category: cchhc zinc binding domain]] | [[Category: cchhc zinc binding domain]] | ||
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[[Category: neural zinc finger factor-1]] | [[Category: neural zinc finger factor-1]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:33:41 2008'' |
Revision as of 12:33, 21 February 2008
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Solution Structure of a CCHHC Domain of Neural Zinc Finger Factor-1
Overview
The structure of a CCHHC zinc-binding domain from neural zinc finger factor-1 (NZF-1) has been determined in solution though the use of NMR methods. This domain is a member of a family of domains that have the Cys-X(4)-Cys-X(4)-His-X(7)-His-X(5)-Cys consensus sequence. The structure determination reveals a novel fold based around a zinc(II) ion coordinated to three Cys residues and the second of the two conserved His residues. The other His residue is stacked between the metal-coordinated His residue and a relatively conserved aromatic residue. Analysis of His to Gln sequence variants reveals that both His residues are required for the formation of a well-defined structure, but neither is required for high-affinity metal binding at a tetrahedral site. The structure suggests that a two-domain protein fragment and a double-stranded DNA binding site may interact with a common two-fold axis relating the two domains and the two half-sites of the DNA-inverted repeat.
About this Structure
1PXE is a Single protein structure of sequence from Rattus norvegicus with as ligand. Full crystallographic information is available from OCA.
Reference
Solution structure of a CCHHC domain of neural zinc finger factor-1 and its implications for DNA binding., Berkovits-Cymet HJ, Amann BT, Berg JM, Biochemistry. 2004 Feb 3;43(4):898-903. PMID:14744132
Page seeded by OCA on Thu Feb 21 14:33:41 2008
