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1pxu
From Proteopedia
(New page: 200px<br /><applet load="1pxu" size="450" color="white" frame="true" align="right" spinBox="true" caption="1pxu, resolution 2.20Å" /> '''Crystal structure of...) |
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| - | [[Image:1pxu.gif|left|200px]]<br /><applet load="1pxu" size=" | + | [[Image:1pxu.gif|left|200px]]<br /><applet load="1pxu" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1pxu, resolution 2.20Å" /> | caption="1pxu, resolution 2.20Å" /> | ||
'''Crystal structure of chicken NtA from a eukaryotic source at 2.2A resolution'''<br /> | '''Crystal structure of chicken NtA from a eukaryotic source at 2.2A resolution'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Agrin is a key organizer for postsynaptic differentiation at the | + | Agrin is a key organizer for postsynaptic differentiation at the neuromuscular junction (NMJ). This activity requires the binding of agrin to the synaptic basal lamina via its N-terminal (NtA) domain. It has been suggested that this binding is mediated by conserved amino acids in the gamma 1 chain of laminin. Here, we report the crystal structure of chicken NtA expressed in eukaryotic HEK293 cells. In contrast to the previously published structure [Stetefeld, J., Jenny, M., Schulthess, T., Landwehr, R., Schumacher, B., Frank, S., Ruegg, M.A., Engel, J., Kammerer, R.A., 2001. The laminin-binding domain of agrin is structurally related to N-TIMP-1. Nat. Struct. Biol., 8, 705-709.], which was derived from the NtA domain expressed in E. coli, the new data show that the N-terminal tail region (amino acid residues Asn1-Arg5) is highly structured. Moreover, the disulfide bridge between Cys2 and Cys74 was also present. In addition, we show that the binding of NtA requires the gamma 1 chain of laminin and is not greatly affected by the composition of beta chains. These results confirm a model of the NtA-laminin complex where conserved amino acids in the gamma 1 chain are prerequisite for the binding to agrin and they further emphasize that the source of protein can be critical in structure determination. |
==About this Structure== | ==About this Structure== | ||
| - | 1PXU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http:// | + | 1PXU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PXU OCA]. |
==Reference== | ==Reference== | ||
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[[Category: agrin]] | [[Category: agrin]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:33:41 2008'' |
Revision as of 12:33, 21 February 2008
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Crystal structure of chicken NtA from a eukaryotic source at 2.2A resolution
Overview
Agrin is a key organizer for postsynaptic differentiation at the neuromuscular junction (NMJ). This activity requires the binding of agrin to the synaptic basal lamina via its N-terminal (NtA) domain. It has been suggested that this binding is mediated by conserved amino acids in the gamma 1 chain of laminin. Here, we report the crystal structure of chicken NtA expressed in eukaryotic HEK293 cells. In contrast to the previously published structure [Stetefeld, J., Jenny, M., Schulthess, T., Landwehr, R., Schumacher, B., Frank, S., Ruegg, M.A., Engel, J., Kammerer, R.A., 2001. The laminin-binding domain of agrin is structurally related to N-TIMP-1. Nat. Struct. Biol., 8, 705-709.], which was derived from the NtA domain expressed in E. coli, the new data show that the N-terminal tail region (amino acid residues Asn1-Arg5) is highly structured. Moreover, the disulfide bridge between Cys2 and Cys74 was also present. In addition, we show that the binding of NtA requires the gamma 1 chain of laminin and is not greatly affected by the composition of beta chains. These results confirm a model of the NtA-laminin complex where conserved amino acids in the gamma 1 chain are prerequisite for the binding to agrin and they further emphasize that the source of protein can be critical in structure determination.
About this Structure
1PXU is a Single protein structure of sequence from Gallus gallus. Full crystallographic information is available from OCA.
Reference
Structure and laminin-binding specificity of the NtA domain expressed in eukaryotic cells., Mascarenhas JB, Ruegg MA, Sasaki T, Eble JA, Engel J, Stetefeld J, Matrix Biol. 2005 Jan;23(8):507-13. Epub 2004 Dec 30. PMID:15694127
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