1py0
From Proteopedia
(New page: 200px<br /><applet load="1py0" size="450" color="white" frame="true" align="right" spinBox="true" caption="1py0, resolution 2.00Å" /> '''Crystal structure of...) |
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| - | [[Image:1py0.gif|left|200px]]<br /><applet load="1py0" size=" | + | [[Image:1py0.gif|left|200px]]<br /><applet load="1py0" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1py0, resolution 2.00Å" /> | caption="1py0, resolution 2.00Å" /> | ||
'''Crystal structure of E51C/E54C Psaz from A.faecalis with CLaNP probe'''<br /> | '''Crystal structure of E51C/E54C Psaz from A.faecalis with CLaNP probe'''<br /> | ||
==Overview== | ==Overview== | ||
| - | A lanthanide complex, named CLaNP (caged lanthanide NMR probe) has been | + | A lanthanide complex, named CLaNP (caged lanthanide NMR probe) has been developed for the characterisation of proteins by paramagnetic NMR spectroscopy. The probe consists of a lanthanide chelated by a derivative of DTPA (diethylenetriaminepentaacetic acid) with two thiol reactive functional groups. The CLaNP molecule is attached to a protein by two engineered, surface-exposed, Cys residues in a bidentate manner. This drastically limits the dynamics of the metal relative to the protein and enables measurements of pseudocontact shifts. NMR spectroscopy experiments on a diamagnetic control and the crystal structure of the probe-protein complex demonstrate that the protein structure is not affected by probe attachment. The probe is able to induce pseudocontact shifts to at least 40 A from the metal and causes residual dipolar couplings due to alignment at a high magnetic field. The molecule exists in several isomeric forms with different paramagnetic tensors; this provides a fast way to obtain long-range distance restraints. |
==About this Structure== | ==About this Structure== | ||
| - | 1PY0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Alcaligenes_faecalis Alcaligenes faecalis] with ZN, Y1, SO4 and YMA as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1PY0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Alcaligenes_faecalis Alcaligenes faecalis] with <scene name='pdbligand=ZN:'>ZN</scene>, <scene name='pdbligand=Y1:'>Y1</scene>, <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=YMA:'>YMA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PY0 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Alcaligenes faecalis]] | [[Category: Alcaligenes faecalis]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Boulanger, M | + | [[Category: Boulanger, M J.]] |
| - | [[Category: Hupkes, H | + | [[Category: Hupkes, H J.]] |
[[Category: Impagliazzo, A.]] | [[Category: Impagliazzo, A.]] | ||
[[Category: Kosters, W.]] | [[Category: Kosters, W.]] | ||
| - | [[Category: Murphy, M | + | [[Category: Murphy, M E.]] |
| - | [[Category: Peters, J | + | [[Category: Peters, J A.]] |
[[Category: Prudencio, M.]] | [[Category: Prudencio, M.]] | ||
[[Category: Rohovec, J.]] | [[Category: Rohovec, J.]] | ||
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[[Category: nmr probe]] | [[Category: nmr probe]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:33:43 2008'' |
Revision as of 12:33, 21 February 2008
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Crystal structure of E51C/E54C Psaz from A.faecalis with CLaNP probe
Overview
A lanthanide complex, named CLaNP (caged lanthanide NMR probe) has been developed for the characterisation of proteins by paramagnetic NMR spectroscopy. The probe consists of a lanthanide chelated by a derivative of DTPA (diethylenetriaminepentaacetic acid) with two thiol reactive functional groups. The CLaNP molecule is attached to a protein by two engineered, surface-exposed, Cys residues in a bidentate manner. This drastically limits the dynamics of the metal relative to the protein and enables measurements of pseudocontact shifts. NMR spectroscopy experiments on a diamagnetic control and the crystal structure of the probe-protein complex demonstrate that the protein structure is not affected by probe attachment. The probe is able to induce pseudocontact shifts to at least 40 A from the metal and causes residual dipolar couplings due to alignment at a high magnetic field. The molecule exists in several isomeric forms with different paramagnetic tensors; this provides a fast way to obtain long-range distance restraints.
About this Structure
1PY0 is a Single protein structure of sequence from Alcaligenes faecalis with , , and as ligands. Full crystallographic information is available from OCA.
Reference
A caged lanthanide complex as a paramagnetic shift agent for protein NMR., Prudencio M, Rohovec J, Peters JA, Tocheva E, Boulanger MJ, Murphy ME, Hupkes HJ, Kosters W, Impagliazzo A, Ubbink M, Chemistry. 2004 Jul 5;10(13):3252-60. PMID:15224334
Page seeded by OCA on Thu Feb 21 14:33:43 2008
Categories: Alcaligenes faecalis | Single protein | Boulanger, M J. | Hupkes, H J. | Impagliazzo, A. | Kosters, W. | Murphy, M E. | Peters, J A. | Prudencio, M. | Rohovec, J. | Tocheva, E. | Ubbink, M. | SO4 | Y1 | YMA | ZN | Cupredoxin | Nmr probe
