1pzb
From Proteopedia
(New page: 200px<br /><applet load="1pzb" size="450" color="white" frame="true" align="right" spinBox="true" caption="1pzb, resolution 1.8Å" /> '''THE CRYSTAL STRUCTURE...) |
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| - | [[Image:1pzb.jpg|left|200px]]<br /><applet load="1pzb" size=" | + | [[Image:1pzb.jpg|left|200px]]<br /><applet load="1pzb" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1pzb, resolution 1.8Å" /> | caption="1pzb, resolution 1.8Å" /> | ||
'''THE CRYSTAL STRUCTURES OF REDUCED PSEUDOAZURIN FROM ALCALIGENES FAECALIS S-6 AT TWO PH VALUES'''<br /> | '''THE CRYSTAL STRUCTURES OF REDUCED PSEUDOAZURIN FROM ALCALIGENES FAECALIS S-6 AT TWO PH VALUES'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The structures of the reduced (Cu1+) blue-copper protein pseudoazurin from | + | The structures of the reduced (Cu1+) blue-copper protein pseudoazurin from Alcaligenes faecalis strain S-6 are refined at pH 7.8 and 4.4 using X-ray diffraction data to 1.8 A resolution. The final R-factors for the high and low pH structures are 0.178 and 0.177, respectively. Comparing the reduced pseudoazurin at pH 7.8 with the oxidised (Cu2+) molecule, small changes are observed in the vicinity of the copper site and on the protein surface. At pH 4.4 the copper substituent imidazole of His81 rotates away from the metal with a concurrent movement of the latter towards the plane of the remaining three ligands (S gamma-Cys78, N delta 1-His40 and S delta-Met86) thus the geometry of the copper site becomes planar trigonal. |
==About this Structure== | ==About this Structure== | ||
| - | 1PZB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Alcaligenes_faecalis Alcaligenes faecalis] with CU as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1PZB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Alcaligenes_faecalis Alcaligenes faecalis] with <scene name='pdbligand=CU:'>CU</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PZB OCA]. |
==Reference== | ==Reference== | ||
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[[Category: electron transfer(cuproprotein)]] | [[Category: electron transfer(cuproprotein)]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:34:10 2008'' |
Revision as of 12:34, 21 February 2008
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THE CRYSTAL STRUCTURES OF REDUCED PSEUDOAZURIN FROM ALCALIGENES FAECALIS S-6 AT TWO PH VALUES
Overview
The structures of the reduced (Cu1+) blue-copper protein pseudoazurin from Alcaligenes faecalis strain S-6 are refined at pH 7.8 and 4.4 using X-ray diffraction data to 1.8 A resolution. The final R-factors for the high and low pH structures are 0.178 and 0.177, respectively. Comparing the reduced pseudoazurin at pH 7.8 with the oxidised (Cu2+) molecule, small changes are observed in the vicinity of the copper site and on the protein surface. At pH 4.4 the copper substituent imidazole of His81 rotates away from the metal with a concurrent movement of the latter towards the plane of the remaining three ligands (S gamma-Cys78, N delta 1-His40 and S delta-Met86) thus the geometry of the copper site becomes planar trigonal.
About this Structure
1PZB is a Single protein structure of sequence from Alcaligenes faecalis with as ligand. Full crystallographic information is available from OCA.
Reference
The crystal structures of reduced pseudoazurin from Alcaligenes faecalis S-6 at two pH values., Vakoufari E, Wilson KS, Petratos K, FEBS Lett. 1994 Jun 27;347(2-3):203-6. PMID:8034003
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