1q5i
From Proteopedia
(New page: 200px<br /><applet load="1q5i" size="450" color="white" frame="true" align="right" spinBox="true" caption="1q5i, resolution 2.3Å" /> '''Crystal structure of ...) |
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| - | [[Image:1q5i.jpg|left|200px]]<br /><applet load="1q5i" size=" | + | [[Image:1q5i.jpg|left|200px]]<br /><applet load="1q5i" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1q5i, resolution 2.3Å" /> | caption="1q5i, resolution 2.3Å" /> | ||
'''Crystal structure of bacteriorhodopsin mutant P186A crystallized from bicelles'''<br /> | '''Crystal structure of bacteriorhodopsin mutant P186A crystallized from bicelles'''<br /> | ||
==Overview== | ==Overview== | ||
| - | One of the hallmarks of membrane protein structure is the high frequency | + | One of the hallmarks of membrane protein structure is the high frequency of transmembrane helix kinks, which commonly occur at proline residues. Because the proline side chain usually precludes normal helix geometry, it is reasonable to expect that proline residues generate these kinks. We observe, however, that the three prolines in bacteriorhodopsin transmembrane helices can be changed to alanine with little structural consequences. This finding leads to a conundrum: if proline is not required for helix bending, why are prolines commonly present at bends in transmembrane helices? We propose an evolutionary hypothesis in which a mutation to proline initially induces the kink. The resulting packing defects are later repaired by further mutation, thereby locking the kink in the structure. Thus, most prolines in extant proteins can be removed without major structural consequences. We further propose that nonproline kinks are places where vestigial prolines were later removed during evolution. Consistent with this hypothesis, at 14 of 17 nonproline kinks in membrane proteins of known structure, we find prolines in homologous sequences. Our analysis allows us to predict kink positions with >90% reliability. Kink prediction indicates that different G protein-coupled receptor proteins have different kink patterns and therefore different structures. |
==About this Structure== | ==About this Structure== | ||
| - | 1Q5I is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Halobacterium_salinarum Halobacterium salinarum] with RET as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1Q5I is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Halobacterium_salinarum Halobacterium salinarum] with <scene name='pdbligand=RET:'>RET</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q5I OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Halobacterium salinarum]] | [[Category: Halobacterium salinarum]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Bowie, J | + | [[Category: Bowie, J U.]] |
[[Category: Faham, S.]] | [[Category: Faham, S.]] | ||
| - | [[Category: Whitelegge, J | + | [[Category: Whitelegge, J P.]] |
[[Category: Yang, D.]] | [[Category: Yang, D.]] | ||
[[Category: Yohannan, S.]] | [[Category: Yohannan, S.]] | ||
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[[Category: membrane protein]] | [[Category: membrane protein]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:36:03 2008'' |
Revision as of 12:36, 21 February 2008
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Crystal structure of bacteriorhodopsin mutant P186A crystallized from bicelles
Overview
One of the hallmarks of membrane protein structure is the high frequency of transmembrane helix kinks, which commonly occur at proline residues. Because the proline side chain usually precludes normal helix geometry, it is reasonable to expect that proline residues generate these kinks. We observe, however, that the three prolines in bacteriorhodopsin transmembrane helices can be changed to alanine with little structural consequences. This finding leads to a conundrum: if proline is not required for helix bending, why are prolines commonly present at bends in transmembrane helices? We propose an evolutionary hypothesis in which a mutation to proline initially induces the kink. The resulting packing defects are later repaired by further mutation, thereby locking the kink in the structure. Thus, most prolines in extant proteins can be removed without major structural consequences. We further propose that nonproline kinks are places where vestigial prolines were later removed during evolution. Consistent with this hypothesis, at 14 of 17 nonproline kinks in membrane proteins of known structure, we find prolines in homologous sequences. Our analysis allows us to predict kink positions with >90% reliability. Kink prediction indicates that different G protein-coupled receptor proteins have different kink patterns and therefore different structures.
About this Structure
1Q5I is a Single protein structure of sequence from Halobacterium salinarum with as ligand. Full crystallographic information is available from OCA.
Reference
The evolution of transmembrane helix kinks and the structural diversity of G protein-coupled receptors., Yohannan S, Faham S, Yang D, Whitelegge JP, Bowie JU, Proc Natl Acad Sci U S A. 2004 Jan 27;101(4):959-63. Epub 2004 Jan 19. PMID:14732697
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