2c54
From Proteopedia
(New page: 200px<br /> <applet load="2c54" size="450" color="white" frame="true" align="right" spinBox="true" caption="2c54, resolution 1.500Å" /> '''GDP-MANNOSE-3', 5'...) |
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==About this Structure== | ==About this Structure== | ||
| - | 2C54 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]] with GKE, NAD, EPE and FMT as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.1.3.18 5.1.3.18]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2C54 OCA]]. | + | 2C54 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]] with GKE, NAD, EPE and FMT as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/GDP-mannose_3,5-epimerase GDP-mannose 3,5-epimerase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.1.3.18 5.1.3.18]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2C54 OCA]]. |
==Reference== | ==Reference== | ||
Structure and function of GDP-mannose-3',5'-epimerase: an enzyme which performs three chemical reactions at the same active site., Major LL, Wolucka BA, Naismith JH, J Am Chem Soc. 2005 Dec 28;127(51):18309-20. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16366586 16366586] | Structure and function of GDP-mannose-3',5'-epimerase: an enzyme which performs three chemical reactions at the same active site., Major LL, Wolucka BA, Naismith JH, J Am Chem Soc. 2005 Dec 28;127(51):18309-20. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16366586 16366586] | ||
[[Category: Arabidopsis thaliana]] | [[Category: Arabidopsis thaliana]] | ||
| + | [[Category: GDP-mannose 3,5-epimerase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Major, L.L.]] | [[Category: Major, L.L.]] | ||
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[[Category: vitamin c]] | [[Category: vitamin c]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 13:37:18 2007'' |
Revision as of 11:32, 30 October 2007
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GDP-MANNOSE-3', 5'-EPIMERASE (ARABIDOPSIS THALIANA), K178R, WITH GDP-BETA-L-GULOSE AND GDP-4-KETO-BETA-L-GULOSE BOUND IN ACTIVE SITE.
Overview
GDP-mannose-3',5'-epimerase (GME) from Arabidopsis thaliana catalyzes the, epimerization of both the 3' and 5' positions of GDP-alpha-D-mannose to, yield GDP-beta-L-galactose. Production of the C5' epimer of, GDP-alpha-D-mannose, GDP-beta-L-gulose, has also been reported. The, reaction occurs as part of vitamin C biosynthesis in plants. We have, determined structures of complexes of GME with GDP-alpha-D-mannose, GDP-beta-L-galactose, and a mixture of GDP-beta-L-gulose with, GDP-beta-L-4-keto-gulose to resolutions varying from 2.0 to 1.4 A. The, enzyme has the classical extended short-chain dehydratase/reductase (SDR), fold. We have confirmed that GME establishes an equilibrium between two, products, GDP-beta-L-galactose and GDP-beta-L-gulose. The reaction, proceeds by C4' oxidation of ... [(full description)]
About this Structure
2C54 is a [Single protein] structure of sequence from [Arabidopsis thaliana] with GKE, NAD, EPE and FMT as [ligands]. Active as [GDP-mannose 3,5-epimerase], with EC number [5.1.3.18]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
Structure and function of GDP-mannose-3',5'-epimerase: an enzyme which performs three chemical reactions at the same active site., Major LL, Wolucka BA, Naismith JH, J Am Chem Soc. 2005 Dec 28;127(51):18309-20. PMID:16366586
Page seeded by OCA on Tue Oct 30 13:37:18 2007
Categories: Arabidopsis thaliana | GDP-mannose 3,5-epimerase | Single protein | Major, L.L. | Naismith, J.H. | Wolucka, B.A. | EPE | FMT | GKE | NAD | 3' 5'-epimerase | Ascorbate biosynthesis | Gdp-galactose | Gdp-gulose | Gdp-mannose | Isomerase | Keto intermediate | Nad | Sdr | Short chain dehydratase/reductase | Vitamin c
