1qfs
From Proteopedia
(New page: 200px<br /><applet load="1qfs" size="450" color="white" frame="true" align="right" spinBox="true" caption="1qfs, resolution 2.0Å" /> '''PROLYL OLIGOPEPTIDASE...) |
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| - | [[Image:1qfs.gif|left|200px]]<br /><applet load="1qfs" size=" | + | [[Image:1qfs.gif|left|200px]]<br /><applet load="1qfs" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1qfs, resolution 2.0Å" /> | caption="1qfs, resolution 2.0Å" /> | ||
'''PROLYL OLIGOPEPTIDASE FROM PORCINE MUSCLE WITH COVALENTLY BOUND INHIBITOR Z-PRO-PROLINAL'''<br /> | '''PROLYL OLIGOPEPTIDASE FROM PORCINE MUSCLE WITH COVALENTLY BOUND INHIBITOR Z-PRO-PROLINAL'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Prolyl oligopeptidase is a large cytosolic enzyme that belongs to a new | + | Prolyl oligopeptidase is a large cytosolic enzyme that belongs to a new class of serine peptidases. The enzyme is involved in the maturation and degradation of peptide hormones and neuropeptides, which relate to the induction of amnesia. The 1.4 A resolution crystal structure is presented here. The enzyme contains a peptidase domain with an alpha/beta hydrolase fold, and its catalytic triad (Ser554, His680, Asp641) is covered by the central tunnel of an unusual beta propeller. This domain makes prolyl oligopeptidase an oligopeptidase by excluding large structured peptides from the active site. In this way, the propeller protects larger peptides and proteins from proteolysis in the cytosol. The structure is also obtained with a transition state inhibitor, which may facilitate drug design to treat memory disorders. |
==About this Structure== | ==About this Structure== | ||
| - | 1QFS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa] with ZPR as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Prolyl_oligopeptidase Prolyl oligopeptidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.26 3.4.21.26] Full crystallographic information is available from [http:// | + | 1QFS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa] with <scene name='pdbligand=ZPR:'>ZPR</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Prolyl_oligopeptidase Prolyl oligopeptidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.26 3.4.21.26] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QFS OCA]. |
==Reference== | ==Reference== | ||
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[[Category: prolyl oligopeptidase]] | [[Category: prolyl oligopeptidase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:39:02 2008'' |
Revision as of 12:39, 21 February 2008
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PROLYL OLIGOPEPTIDASE FROM PORCINE MUSCLE WITH COVALENTLY BOUND INHIBITOR Z-PRO-PROLINAL
Overview
Prolyl oligopeptidase is a large cytosolic enzyme that belongs to a new class of serine peptidases. The enzyme is involved in the maturation and degradation of peptide hormones and neuropeptides, which relate to the induction of amnesia. The 1.4 A resolution crystal structure is presented here. The enzyme contains a peptidase domain with an alpha/beta hydrolase fold, and its catalytic triad (Ser554, His680, Asp641) is covered by the central tunnel of an unusual beta propeller. This domain makes prolyl oligopeptidase an oligopeptidase by excluding large structured peptides from the active site. In this way, the propeller protects larger peptides and proteins from proteolysis in the cytosol. The structure is also obtained with a transition state inhibitor, which may facilitate drug design to treat memory disorders.
About this Structure
1QFS is a Single protein structure of sequence from Sus scrofa with as ligand. Active as Prolyl oligopeptidase, with EC number 3.4.21.26 Full crystallographic information is available from OCA.
Reference
Prolyl oligopeptidase: an unusual beta-propeller domain regulates proteolysis., Fulop V, Bocskei Z, Polgar L, Cell. 1998 Jul 24;94(2):161-70. PMID:9695945
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