1qk7
From Proteopedia
(New page: 200px<br /><applet load="1qk7" size="450" color="white" frame="true" align="right" spinBox="true" caption="1qk7" /> '''SOLUTION STRUCTURE OF SELENOCOSMIA HUWENA LE...) |
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| - | [[Image:1qk7.jpg|left|200px]]<br /><applet load="1qk7" size=" | + | [[Image:1qk7.jpg|left|200px]]<br /><applet load="1qk7" size="350" color="white" frame="true" align="right" spinBox="true" |
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'''SOLUTION STRUCTURE OF SELENOCOSMIA HUWENA LECTIN-I(SHL-I) BY 2D-NMR'''<br /> | '''SOLUTION STRUCTURE OF SELENOCOSMIA HUWENA LECTIN-I(SHL-I) BY 2D-NMR'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The three-dimensional structure of native SHL-I, a lectin from the venom | + | The three-dimensional structure of native SHL-I, a lectin from the venom of the Chinese bird spider Selenocosmia huwena, has been determined from two-dimensional 1H NMR spectroscopy recorded at 500 and 600 MHz. The best 10 structures have NOE violation <0.3 A, dihedral violation <2 deg, and average root-mean-square differences of 0.85 + 0.06 A over backbone atoms. The structure consists of a three-stranded antiparallel beta-sheet and three turns. The three disulfide bridges and three-stranded antiparallel beta-sheet form a inhibitor cystine knot motif which is adopted by several other small proteins, such as huwentoxin-I, omega-conotoxin, and gurmarin. The C-terminal fragment from Leu28 to Trp32 adopts two sets of conformations corresponding to the cis and trans conformations of Pro31. The structure of SHL-I also has high similarity with that of the N-terminus of hevein, a lectin from rubber-tree latex. |
==About this Structure== | ==About this Structure== | ||
| - | 1QK7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Ornithoctonus_huwena Ornithoctonus huwena]. Full crystallographic information is available from [http:// | + | 1QK7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Ornithoctonus_huwena Ornithoctonus huwena]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QK7 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: shl-i]] | [[Category: shl-i]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:40:33 2008'' |
Revision as of 12:40, 21 February 2008
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SOLUTION STRUCTURE OF SELENOCOSMIA HUWENA LECTIN-I(SHL-I) BY 2D-NMR
Overview
The three-dimensional structure of native SHL-I, a lectin from the venom of the Chinese bird spider Selenocosmia huwena, has been determined from two-dimensional 1H NMR spectroscopy recorded at 500 and 600 MHz. The best 10 structures have NOE violation <0.3 A, dihedral violation <2 deg, and average root-mean-square differences of 0.85 + 0.06 A over backbone atoms. The structure consists of a three-stranded antiparallel beta-sheet and three turns. The three disulfide bridges and three-stranded antiparallel beta-sheet form a inhibitor cystine knot motif which is adopted by several other small proteins, such as huwentoxin-I, omega-conotoxin, and gurmarin. The C-terminal fragment from Leu28 to Trp32 adopts two sets of conformations corresponding to the cis and trans conformations of Pro31. The structure of SHL-I also has high similarity with that of the N-terminus of hevein, a lectin from rubber-tree latex.
About this Structure
1QK7 is a Single protein structure of sequence from Ornithoctonus huwena. Full crystallographic information is available from OCA.
Reference
Three-dimensional structure of Selenocosmia huwena lectin-I (SHL-I) from the venom of the spider Selenocosmia huwena by 2D-NMR., Lu S, Liang S, Gu X, J Protein Chem. 1999 Jul;18(5):609-17. PMID:10524779
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