1qu9
From Proteopedia
(New page: 200px<br /><applet load="1qu9" size="450" color="white" frame="true" align="right" spinBox="true" caption="1qu9, resolution 1.2Å" /> '''1.2 A CRYSTAL STRUCTU...) |
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| - | [[Image:1qu9.gif|left|200px]]<br /><applet load="1qu9" size=" | + | [[Image:1qu9.gif|left|200px]]<br /><applet load="1qu9" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1qu9, resolution 1.2Å" /> | caption="1qu9, resolution 1.2Å" /> | ||
'''1.2 A CRYSTAL STRUCTURE OF YJGF GENE PRODUCT FROM E. COLI'''<br /> | '''1.2 A CRYSTAL STRUCTURE OF YJGF GENE PRODUCT FROM E. COLI'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The YER057c/YIL051c/YjgF protein family is a set of 24 full-length | + | The YER057c/YIL051c/YjgF protein family is a set of 24 full-length homologs, each approximately 130 residues in length, and each with no known function or relationship to proteins of known structure. To determine the function of this family, the structure of one member--the YjgF protein from Escherichia coli--was solved and refined at a resolution of 1.2 A. The YjgF molecule is a homotrimer with exact threefold symmetry. Its tertiary and quaternary structures are related to that of Bacillus subtilis chorismate mutase, although their active sites are completely different. The YjgF protein has an active site curiously similar to protein tyrosine phosphatases, including a covalently modified cysteine, but it is unlikely to be functionally related. The lessons learned from this attempt to deduce function from structure may be useful to future projects in structural genomics. |
==About this Structure== | ==About this Structure== | ||
| - | 1QU9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http:// | + | 1QU9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QU9 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: structural genomics]] | [[Category: structural genomics]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:43:46 2008'' |
Revision as of 12:43, 21 February 2008
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1.2 A CRYSTAL STRUCTURE OF YJGF GENE PRODUCT FROM E. COLI
Overview
The YER057c/YIL051c/YjgF protein family is a set of 24 full-length homologs, each approximately 130 residues in length, and each with no known function or relationship to proteins of known structure. To determine the function of this family, the structure of one member--the YjgF protein from Escherichia coli--was solved and refined at a resolution of 1.2 A. The YjgF molecule is a homotrimer with exact threefold symmetry. Its tertiary and quaternary structures are related to that of Bacillus subtilis chorismate mutase, although their active sites are completely different. The YjgF protein has an active site curiously similar to protein tyrosine phosphatases, including a covalently modified cysteine, but it is unlikely to be functionally related. The lessons learned from this attempt to deduce function from structure may be useful to future projects in structural genomics.
About this Structure
1QU9 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
A test case for structure-based functional assignment: the 1.2 A crystal structure of the yjgF gene product from Escherichia coli., Volz K, Protein Sci. 1999 Nov;8(11):2428-37. PMID:10595546
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