1qxw
From Proteopedia
(New page: 200px<br /><applet load="1qxw" size="450" color="white" frame="true" align="right" spinBox="true" caption="1qxw, resolution 1.67Å" /> '''Crystal structure of...) |
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| - | [[Image:1qxw.jpg|left|200px]]<br /><applet load="1qxw" size=" | + | [[Image:1qxw.jpg|left|200px]]<br /><applet load="1qxw" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1qxw, resolution 1.67Å" /> | caption="1qxw, resolution 1.67Å" /> | ||
'''Crystal structure of Staphyloccocus aureus in complex with an aminoketone inhibitor 54135.'''<br /> | '''Crystal structure of Staphyloccocus aureus in complex with an aminoketone inhibitor 54135.'''<br /> | ||
==Overview== | ==Overview== | ||
| - | High-resolution crystal structures of Staphylococcus aureus methionine | + | High-resolution crystal structures of Staphylococcus aureus methionine aminopeptidase I in complex with various keto heterocycles and aminoketones were determined, and the intermolecular ligand interactions with the enzyme are reported. The compounds are effective inhibitors of the S. aureus enzyme because of the formation of an uncleavable tetrahedral intermediate upon binding. The electron densities unequivocally show the enzyme-catalyzed transition-state analogue mimicking that for amide bond hydrolysis of substrates. |
==About this Structure== | ==About this Structure== | ||
| - | 1QXW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus] with CO, ACT and M1C as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Methionyl_aminopeptidase Methionyl aminopeptidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.11.18 3.4.11.18] Full crystallographic information is available from [http:// | + | 1QXW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus] with <scene name='pdbligand=CO:'>CO</scene>, <scene name='pdbligand=ACT:'>ACT</scene> and <scene name='pdbligand=M1C:'>M1C</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Methionyl_aminopeptidase Methionyl aminopeptidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.11.18 3.4.11.18] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QXW OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Staphylococcus aureus]] | [[Category: Staphylococcus aureus]] | ||
| - | [[Category: Arcy, A | + | [[Category: Arcy, A D.]] |
| - | [[Category: Dale, G | + | [[Category: Dale, G E.]] |
[[Category: Douangamath, A.]] | [[Category: Douangamath, A.]] | ||
[[Category: Oefner, C.]] | [[Category: Oefner, C.]] | ||
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[[Category: pita bread fold]] | [[Category: pita bread fold]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:44:53 2008'' |
Revision as of 12:44, 21 February 2008
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Crystal structure of Staphyloccocus aureus in complex with an aminoketone inhibitor 54135.
Overview
High-resolution crystal structures of Staphylococcus aureus methionine aminopeptidase I in complex with various keto heterocycles and aminoketones were determined, and the intermolecular ligand interactions with the enzyme are reported. The compounds are effective inhibitors of the S. aureus enzyme because of the formation of an uncleavable tetrahedral intermediate upon binding. The electron densities unequivocally show the enzyme-catalyzed transition-state analogue mimicking that for amide bond hydrolysis of substrates.
About this Structure
1QXW is a Single protein structure of sequence from Staphylococcus aureus with , and as ligands. Active as Methionyl aminopeptidase, with EC number 3.4.11.18 Full crystallographic information is available from OCA.
Reference
Crystal structures of Staphylococcusaureus methionine aminopeptidase complexed with keto heterocycle and aminoketone inhibitors reveal the formation of a tetrahedral intermediate., Douangamath A, Dale GE, D'Arcy A, Almstetter M, Eckl R, Frutos-Hoener A, Henkel B, Illgen K, Nerdinger S, Schulz H, Mac Sweeney A, Thormann M, Treml A, Pierau S, Wadman S, Oefner C, J Med Chem. 2004 Mar 11;47(6):1325-8. PMID:14998322
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