1r11
From Proteopedia
(New page: 200px<br /><applet load="1r11" size="450" color="white" frame="true" align="right" spinBox="true" caption="1r11, resolution 2.70Å" /> '''Structure Determinat...) |
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| - | [[Image:1r11.jpg|left|200px]]<br /><applet load="1r11" size=" | + | [[Image:1r11.jpg|left|200px]]<br /><applet load="1r11" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1r11, resolution 2.70Å" /> | caption="1r11, resolution 2.70Å" /> | ||
'''Structure Determination of the Dimeric Endonuclease in a Pseudo-face-centerd P21 space group'''<br /> | '''Structure Determination of the Dimeric Endonuclease in a Pseudo-face-centerd P21 space group'''<br /> | ||
==Overview== | ==Overview== | ||
| - | RNA-splicing endonuclease is responsible for the excision of introns in | + | RNA-splicing endonuclease is responsible for the excision of introns in transfer RNA and archaeal ribosomal RNAs. The archaeal form of the enzyme recognizes a unique RNA motif that consists of two three-nucleotide bulges separated by a four base-paired helix, known as the bulge-helix-bulge (BHB) motif. A crystal structure of the RNA-splicing endonuclease from Archaeoglobus fulgidus (AF) has been reported previously at 2.8 A. A truncated but fully active form of AF endonuclease that lacks the N-terminal domain was expressed and crystallized in an orthorhombic space group with two dimers in the asymmetric unit. The calculated native Patterson map suggests strong pseudo-face-centering characteristics, which lead to incorrect space-group assignment by the autoindexing program. The correct space group was determined to be P2(1)2(1)2 after reindexing. The structure was solved using molecular replacement and was refined to 2.0 A. The truncated AF endonuclease structure is essentially identical to the corresponding portion of the wild-type AF endonuclease structure in space group P4(3)2(1)2 as reported previously, with the exception of loop L9, which differs owing to different crystallographic packing. These results confirm the previously described structural features of dimeric splicing endonuclease. |
==About this Structure== | ==About this Structure== | ||
| - | 1R11 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Active as [http://en.wikipedia.org/wiki/tRNA-intron_endonuclease tRNA-intron endonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.9 3.1.27.9] Full crystallographic information is available from [http:// | + | 1R11 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Active as [http://en.wikipedia.org/wiki/tRNA-intron_endonuclease tRNA-intron endonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.9 3.1.27.9] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R11 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: rna splicing; endonuclease; x-ray crystallography]] | [[Category: rna splicing; endonuclease; x-ray crystallography]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:45:55 2008'' |
Revision as of 12:45, 21 February 2008
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Structure Determination of the Dimeric Endonuclease in a Pseudo-face-centerd P21 space group
Overview
RNA-splicing endonuclease is responsible for the excision of introns in transfer RNA and archaeal ribosomal RNAs. The archaeal form of the enzyme recognizes a unique RNA motif that consists of two three-nucleotide bulges separated by a four base-paired helix, known as the bulge-helix-bulge (BHB) motif. A crystal structure of the RNA-splicing endonuclease from Archaeoglobus fulgidus (AF) has been reported previously at 2.8 A. A truncated but fully active form of AF endonuclease that lacks the N-terminal domain was expressed and crystallized in an orthorhombic space group with two dimers in the asymmetric unit. The calculated native Patterson map suggests strong pseudo-face-centering characteristics, which lead to incorrect space-group assignment by the autoindexing program. The correct space group was determined to be P2(1)2(1)2 after reindexing. The structure was solved using molecular replacement and was refined to 2.0 A. The truncated AF endonuclease structure is essentially identical to the corresponding portion of the wild-type AF endonuclease structure in space group P4(3)2(1)2 as reported previously, with the exception of loop L9, which differs owing to different crystallographic packing. These results confirm the previously described structural features of dimeric splicing endonuclease.
About this Structure
1R11 is a Single protein structure of sequence from Archaeoglobus fulgidus. Active as tRNA-intron endonuclease, with EC number 3.1.27.9 Full crystallographic information is available from OCA.
Reference
Structure determination of a truncated dimeric splicing endonuclease in pseudo-face-centered space group P2(1)2(1)2., Zhang Y, Li H, Acta Crystallogr D Biol Crystallogr. 2004 Mar;60(Pt 3):447-52. Epub 2004, Feb 25. PMID:14993668
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