1r8n
From Proteopedia
(New page: 200px<br /><applet load="1r8n" size="450" color="white" frame="true" align="right" spinBox="true" caption="1r8n, resolution 1.75Å" /> '''The Crystal Structur...) |
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| - | [[Image:1r8n.jpg|left|200px]]<br /><applet load="1r8n" size=" | + | [[Image:1r8n.jpg|left|200px]]<br /><applet load="1r8n" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1r8n, resolution 1.75Å" /> | caption="1r8n, resolution 1.75Å" /> | ||
'''The Crystal Structure of the Kunitz (STI) Type Inhibitor from Seeds of Delonix regia'''<br /> | '''The Crystal Structure of the Kunitz (STI) Type Inhibitor from Seeds of Delonix regia'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The three-dimensional structure of a novel Kunitz (STI) family member, an | + | The three-dimensional structure of a novel Kunitz (STI) family member, an inhibitor purified from Delonix regia seeds (DrTI), was solved by molecular replacement method and refined, respectively, to R(factor) and R(free) values of 21.5% and 25.3% at 1.75A resolution. The structure has a classical beta-trefoil fold, however, differently from canonical Kunitz type (STI) inhibitors, its reactive site loop has an insertion of one residue, Glu68, between the residues P1 and P2. Surprisingly, DrTI is an effective inhibitor of trypsin and human plasma kallikrein, but not of chymotrypsin and tissue kallikrein. Putative structural grounds of such specificity are discussed. |
==About this Structure== | ==About this Structure== | ||
| - | 1R8N is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Delonix_regia Delonix regia]. Full crystallographic information is available from [http:// | + | 1R8N is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Delonix_regia Delonix regia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R8N OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Krauchenco, S.]] | [[Category: Krauchenco, S.]] | ||
[[Category: Marangoni, S.]] | [[Category: Marangoni, S.]] | ||
| - | [[Category: Pando, S | + | [[Category: Pando, S C.]] |
[[Category: Polikarpov, I.]] | [[Category: Polikarpov, I.]] | ||
[[Category: beta-trefoil fold]] | [[Category: beta-trefoil fold]] | ||
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[[Category: x-ray structure]] | [[Category: x-ray structure]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:48:16 2008'' |
Revision as of 12:48, 21 February 2008
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The Crystal Structure of the Kunitz (STI) Type Inhibitor from Seeds of Delonix regia
Overview
The three-dimensional structure of a novel Kunitz (STI) family member, an inhibitor purified from Delonix regia seeds (DrTI), was solved by molecular replacement method and refined, respectively, to R(factor) and R(free) values of 21.5% and 25.3% at 1.75A resolution. The structure has a classical beta-trefoil fold, however, differently from canonical Kunitz type (STI) inhibitors, its reactive site loop has an insertion of one residue, Glu68, between the residues P1 and P2. Surprisingly, DrTI is an effective inhibitor of trypsin and human plasma kallikrein, but not of chymotrypsin and tissue kallikrein. Putative structural grounds of such specificity are discussed.
About this Structure
1R8N is a Single protein structure of sequence from Delonix regia. Full crystallographic information is available from OCA.
Reference
Crystal structure of the Kunitz (STI)-type inhibitor from Delonix regia seeds., Krauchenco S, Pando SC, Marangoni S, Polikarpov I, Biochem Biophys Res Commun. 2003 Dec 26;312(4):1303-8. PMID:14652016
Page seeded by OCA on Thu Feb 21 14:48:16 2008
