1rb7
From Proteopedia
(New page: 200px<br /><applet load="1rb7" size="450" color="white" frame="true" align="right" spinBox="true" caption="1rb7, resolution 2.10Å" /> '''Yeast cytosine deami...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1rb7.gif|left|200px]]<br /><applet load="1rb7" size=" | + | [[Image:1rb7.gif|left|200px]]<br /><applet load="1rb7" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1rb7, resolution 2.10Å" /> | caption="1rb7, resolution 2.10Å" /> | ||
'''Yeast cytosine deaminase crystal form p212121 with sodium acetate.'''<br /> | '''Yeast cytosine deaminase crystal form p212121 with sodium acetate.'''<br /> | ||
==Overview== | ==Overview== | ||
| - | A crystallization strategy termed 'microseed matrix screening' is | + | A crystallization strategy termed 'microseed matrix screening' is described where the optimal conditions for nucleation versus extended lattice growth are not compatible. This method is an extension of conventional seeding techniques in which microseeds from the nucleation step are systematically seeded into new conditions where all components of the drop are allowed to vary to screen for subsequent growth of well ordered specimens. The structure of a crystal form of yeast cytosine deaminase produced by streak-seeding using a single condition for both nucleation and growth is compared with the structure of a related crystal form produced by separating nucleation and growth conditions. The resulting structural comparison demonstrates that differential chelation patterns of cations by acidic surface residues of proteins within crystal lattice contacts is a critical parameter of crystal nucleation and growth. |
==About this Structure== | ==About this Structure== | ||
| - | 1RB7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Cytosine_deaminase Cytosine deaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.4.1 3.5.4.1] Full crystallographic information is available from [http:// | + | 1RB7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Cytosine_deaminase Cytosine deaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.4.1 3.5.4.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RB7 OCA]. |
==Reference== | ==Reference== | ||
| Line 14: | Line 14: | ||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Ireton, G | + | [[Category: Ireton, G C.]] |
| - | [[Category: Stoddard, B | + | [[Category: Stoddard, B L.]] |
[[Category: ZN]] | [[Category: ZN]] | ||
[[Category: amino hydrolase]] | [[Category: amino hydrolase]] | ||
| Line 22: | Line 22: | ||
[[Category: zinc bound]] | [[Category: zinc bound]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:48:59 2008'' |
Revision as of 12:49, 21 February 2008
|
Yeast cytosine deaminase crystal form p212121 with sodium acetate.
Overview
A crystallization strategy termed 'microseed matrix screening' is described where the optimal conditions for nucleation versus extended lattice growth are not compatible. This method is an extension of conventional seeding techniques in which microseeds from the nucleation step are systematically seeded into new conditions where all components of the drop are allowed to vary to screen for subsequent growth of well ordered specimens. The structure of a crystal form of yeast cytosine deaminase produced by streak-seeding using a single condition for both nucleation and growth is compared with the structure of a related crystal form produced by separating nucleation and growth conditions. The resulting structural comparison demonstrates that differential chelation patterns of cations by acidic surface residues of proteins within crystal lattice contacts is a critical parameter of crystal nucleation and growth.
About this Structure
1RB7 is a Single protein structure of sequence from Saccharomyces cerevisiae with as ligand. Active as Cytosine deaminase, with EC number 3.5.4.1 Full crystallographic information is available from OCA.
Reference
Microseed matrix screening to improve crystals of yeast cytosine deaminase., Ireton GC, Stoddard BL, Acta Crystallogr D Biol Crystallogr. 2004 Mar;60(Pt 3):601-5. Epub 2004, Feb 25. PMID:14993707
Page seeded by OCA on Thu Feb 21 14:48:59 2008
