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1rbj
From Proteopedia
(New page: 200px<br /><applet load="1rbj" size="450" color="white" frame="true" align="right" spinBox="true" caption="1rbj, resolution 2.700Å" /> '''RIBONUCLEASE B COMP...) |
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| - | [[Image:1rbj.gif|left|200px]]<br /><applet load="1rbj" size=" | + | [[Image:1rbj.gif|left|200px]]<br /><applet load="1rbj" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1rbj, resolution 2.700Å" /> | caption="1rbj, resolution 2.700Å" /> | ||
'''RIBONUCLEASE B COMPLEX WITH D(TETRA-(DEOXY-ADENYLATE))'''<br /> | '''RIBONUCLEASE B COMPLEX WITH D(TETRA-(DEOXY-ADENYLATE))'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The structure of a tetragonal crystal of bovine pancreatic RNase B | + | The structure of a tetragonal crystal of bovine pancreatic RNase B complexed with d(pA)(4) was determined by molecular replacement and difference Fourier methods. This crystal belongs to space group P4(1)2(1)2 and has unit-cell dimensions a = b = 44.5, c = 156.5 A. The model consists of the enzyme and a tetranucleotide with fractional occupancies, suggesting multiple modes of oligonucleotide binding. It does not include any polysaccharide residues or solvent molecules. After refinement at 2.7 A, the R value was 0.163 with acceptable stereochemistry. The model illustrates a set of well defined interactions for substrate binding, particularly between the central dinucleotide and the enzyme. |
==About this Structure== | ==About this Structure== | ||
| - | 1RBJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Active as [http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] Full crystallographic information is available from [http:// | + | 1RBJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Active as [http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RBJ OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Pancreatic ribonuclease]] | [[Category: Pancreatic ribonuclease]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Ko, T | + | [[Category: Ko, T P.]] |
[[Category: McPherson, A.]] | [[Category: McPherson, A.]] | ||
[[Category: Williams, R.]] | [[Category: Williams, R.]] | ||
[[Category: protein-dna complex]] | [[Category: protein-dna complex]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:49:15 2008'' |
Revision as of 12:49, 21 February 2008
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RIBONUCLEASE B COMPLEX WITH D(TETRA-(DEOXY-ADENYLATE))
Overview
The structure of a tetragonal crystal of bovine pancreatic RNase B complexed with d(pA)(4) was determined by molecular replacement and difference Fourier methods. This crystal belongs to space group P4(1)2(1)2 and has unit-cell dimensions a = b = 44.5, c = 156.5 A. The model consists of the enzyme and a tetranucleotide with fractional occupancies, suggesting multiple modes of oligonucleotide binding. It does not include any polysaccharide residues or solvent molecules. After refinement at 2.7 A, the R value was 0.163 with acceptable stereochemistry. The model illustrates a set of well defined interactions for substrate binding, particularly between the central dinucleotide and the enzyme.
About this Structure
1RBJ is a Single protein structure of sequence from Bos taurus. Active as Pancreatic ribonuclease, with EC number 3.1.27.5 Full crystallographic information is available from OCA.
Reference
Structure of a ribonuclease B+d(pA)4 complex., Ko TP, Williams R, McPherson A, Acta Crystallogr D Biol Crystallogr. 1996 Jan 1;52(Pt 1):160-4. PMID:15299737
Page seeded by OCA on Thu Feb 21 14:49:15 2008
